INCENP antibody (AA 369-583)

Catalog No. ABIN969529

Product Details anti-INCENP Antibody

Target: INCENP (Inner Centromere Protein Antigens 135/155kDa (INCENP))

Binding Specificity: AA 369-583

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This INCENP antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purpose: INCENP Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.

Clone: 3D2

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-INCENP antibody (ABIN969529)

Ban, Nishida, Urano: "Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 16, Issue 6, pp. 652-69, (2011) (PubMed).

Xu, Ogawa, Vagnarelli, Bergmann, Hudson, Ruchaud, Fukagawa, Earnshaw, Samejima: "INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization." in: The Journal of cell biology, Vol. 187, Issue 5, pp. 637-53, (2009) (PubMed).

Target Details for INCENP

Target: INCENP (Inner Centromere Protein Antigens 135/155kDa (INCENP))

Alternative Name: INCENP (INCENP Products)

Synonyms: INCENP antibody, LOC572121 antibody, 2700067E22Rik antibody, AU019509 antibody, C130081E20 antibody, C77457 antibody, XL-INCENP antibody, incenp antibody, incenp-a antibody, incenp-b antibody, xINC antibody, xIncenp antibody, xl-incenp antibody, inner centromere protein antibody, inner centromere protein S homeolog antibody, inner centromere protein L homeolog antibody, INCENP antibody, Incenp antibody, incenp.S antibody, incenp antibody, incenp.L antibody

Background: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A, MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143), MCAK (MIM 604538), KID (MIM 603213), cytoplasmic dynein (e.g., MIM 600112), CliPs (e.g., MIM 179838), and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis

Molecular Weight: 105.4kDa

Gene ID: 3619

HGNC: 3619

UniProt: Q9NQS7

Pathways: Cell Division Cycle