E2F1 antibody (AA 69-223)

Catalog No. ABIN969516

Product Details anti-E2F1 Antibody

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Binding Specificity: AA 69-223

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: E2F1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.

Clone: 8G9

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-E2F1 antibody (ABIN969516)

Zhou, Jiang, Xia, Zhang: "Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo." in: International journal of oncology, Vol. 57, Issue 3, pp. 697-706, (2020) (PubMed).

Okazaki, Matsunaga, Okazaki, Utoguchi, Suzuki, Maruyama, Koyanagi, Ohdo: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." in: Cancer research, Vol. 70, Issue 15, pp. 6238-46, (2010) (PubMed).

Gurtner, Fuschi, Martelli, Manni, Artuso, Simonte, Ambrosino, Antonini, Folgiero, Falcioni, Sacchi, Piaggio: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." in: Cancer research, Vol. 70, Issue 23, pp. 9711-20, (2010) (PubMed).

Locatelli, Bosotti, Ciomei, Brasca, Calogero, Mercurio, Fiorentini, Bertolotti, Scacheri, Scaburri, Galvani, Pesenti, De Baere, Soria, Lazar, Isacchi: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." in: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, (2010) (PubMed).

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1 (E2F1 Products)

Synonyms: CG6376 antibody, DRTF1/E2F antibody, DmE2F-1 antibody, Dmel\\CG6376 antibody, Dp antibody, E(Sev-CycE)3A antibody, E(var)3-93E antibody, E(var)3-95E antibody, E(var)93E antibody, E2F antibody, E2F-1 antibody, E2F1 antibody, E2f1 antibody, Evar(3)164 antibody, dE2F antibody, dE2F1 antibody, dE2f antibody, dE2f1 antibody, de2f1 antibody, def21 antibody, drosE2F1 antibody, e2f antibody, e2f1 antibody, l(3)07172 antibody, l(3)j3B1 antibody, l(3)j3C2 antibody, l(3)rM729 antibody, RBAP1 antibody, RBBP3 antibody, RBP3 antibody, mKIAA4009 antibody, xE2F antibody, ATE2FB antibody, E2F TRANSCRIPTION FACTOR-1 E2F1 antibody, E2F transcription factor 1 antibody, E2FB antibody, E2FB TRANSCRIPTION FACTOR antibody, E2F transcription factor 1 antibody, E2F transcription factor 1 L homeolog antibody, E2f1 antibody, E2F1 antibody, e2f1.L antibody

Background:

Description: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Aliases: RBP3, E2F-1, RBAP1, RBBP3

Molecular Weight: 46.9kDa

Gene ID: 1869

HGNC: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy