Doublecortin antibody (AA 362-411)

Catalog No. ABIN969513

Product Details anti-Doublecortin Antibody

Target: Doublecortin (DCX)

Binding Specificity: AA 362-411

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: DCX Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human DCX (AA: 362-411) expressed in E. Coli.

Clone: 2G5

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Doublecortin antibody (ABIN969513)

Evangelisti, Florian, Massimi, Dominici, Giannini, Galardi, Buè, Massalini, McDowell, Messi, Gulino, Farace, Ciafrè: "MiR-128 up-regulation inhibits Reelin and DCX expression and reduces neuroblastoma cell motility and invasiveness." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 12, pp. 4276-87, (2009) (PubMed).

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX (DCX Products)

Synonyms: DCX antibody, DBCN antibody, DC antibody, LISX antibody, SCLH antibody, XLIS antibody, Dbct antibody, 18C15.5 antibody, doublecortin antibody, DCX antibody, Dcx antibody

Background:

Description: This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.

Aliases: DC, DBCN, LISX, SCLH, XLIS

Molecular Weight: 49.3kDa

Gene ID: 1641

HGNC: 1641

UniProt: O43602