Mouse anti-Human IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN969481

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Characteristics: Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Purification: Purified

Immunogen: Human IgG was isolated from human sera and purified by chromatography.

Clone: 4D2D9G8-4A10

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C or at -20 °C for long term.

References

Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, (1996) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Molecular Weight: 50 kDa