SOX2 antibody

Catalog No. ABIN969414

Product Details anti-SOX2 Antibody

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: SOX2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human SOX2 expressed in E. Coli.

Clone: 10F10

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SOX2 antibody (ABIN969414)

Dabdoub, Puligilla, Jones, Fritzsch, Cheah, Pevny, Kelley: "Sox2 signaling in prosensory domain specification and subsequent hair cell differentiation in the developing cochlea." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18396-401, (2008) (PubMed).

Qin, Gan, Shao, Wang, Li, Wang, He, Xu, Zhang, Kou, Zeng, Sheng, Esteban, Gao, Pei: "Mouse meningiocytes express Sox2 and yield high efficiency of chimeras after nuclear reprogramming with exogenous factors." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33730-5, (2008) (PubMed).

Tay, Zhang, Thomson, Lim, Rigoutsos: "MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation." in: Nature, Vol. 455, Issue 7216, pp. 1124-8, (2008) (PubMed).

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2 (SOX2 Products)

Background:

Description: This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Aliases: ANOP3, MCOPS3

Molecular Weight: 34kDa

Gene ID: 6657

HGNC: 20674

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis