Prohibitin antibody

Catalog No. ABIN969353

Product Details anti-Prohibitin Antibody

Target: Prohibitin (PHB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: PHB Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human PHB expressed in E. Coli.

Clone: 5H7

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Prohibitin antibody (ABIN969353)

Shi, Li, Liu, Xu, Tang, Liang, Zhao, Yang: "Nuclear matrix protein, prohibitin, was down-regulated and translocated from nucleus to cytoplasm during the differentiation of osteosarcoma MG-63 cells induced by ginsenoside Rg1, cinnamic acid, and tanshinone IIA (RCT)." in: Journal of cellular biochemistry, Vol. 108, Issue 4, pp. 926-34, (2009) (PubMed).

Ande, Mishra: "Prohibitin interacts with phosphatidylinositol 3,4,5-triphosphate (PIP3) and modulates insulin signaling." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 1023-8, (2009) (PubMed).

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: PHB (PHB Products)

Synonyms: phb1 antibody, MGC75944 antibody, PHB antibody, Phb antibody, ACYPI000080 antibody, phb antibody, PHB1 antibody, Bap32 antibody, sr:nyz128 antibody, wu:fa18f07 antibody, wu:fb09f05 antibody, prohibitin1 antibody, xphb1 antibody, prohibitin antibody, prohibitin-2 antibody, prohibitin L homeolog antibody, PHB antibody, phb antibody, LOC484431 antibody, LOC5576054 antibody, Phb antibody, phb.L antibody

Background:

Description: Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

Aliases: PHB1

Molecular Weight: 30kDa

Gene ID: 5245

HGNC: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling