OCT4 antibody (AA 193-360)

Catalog No. ABIN969327

Product Details anti-OCT4 Antibody

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Binding Specificity: AA 193-360

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: ELISA

Purpose: 5-Oct Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of Oct4 (aa193-360) expressed in E. Coli.

Clone: 1D10H6

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-OCT4 antibody (ABIN969327)

Kerkis, Kerkis, Dozortsev, Stukart-Parsons, Gomes Massironi, Pereira, Caplan, Cerruti: "Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers." in: Cells, tissues, organs, Vol. 184, Issue 3-4, pp. 105-16, (2007) (PubMed).

Atlasi, Mowla, Ziaee, Bahrami: "OCT-4, an embryonic stem cell marker, is highly expressed in bladder cancer." in: International journal of cancer. Journal international du cancer, Vol. 120, Issue 7, pp. 1598-602, (2007) (PubMed).

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct4 (POU5F1 Products)

Background:

Description: Oct4: octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, also known as POU5F1(POU class 5 homeobox 1), octamerbinding transcription factor-3 (Oct-3, Otf-3), modulates embryonic stem (ES) cell populations by influencing lineage commitment. Entrez Protein NP_002692. Oct-3/4 sustains stem-cell selfrenewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.

Aliases: OCT3, OTF3, OTF4, MGC22487, POU5F1

Molecular Weight: 38.5kDa

Gene ID: 5460

HGNC: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance