NQO1 antibody

Catalog No. ABIN969319

Product Details anti-NQO1 Antibody

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NQO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: NQO1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human NQO1 expressed in E. Coli.

Clone: 4D12

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-NQO1 antibody (ABIN969319)

Kansanen, Jyrkkänen, Volger, Leinonen, Kivelä, Häkkinen, Woodcock, Schopfer, Horrevoets, Ylä-Herttuala, Freeman, Levonen: "Nrf2-dependent and -independent responses to nitro-fatty acids in human endothelial cells: identification of heat shock response as the major pathway activated by nitro-oleic acid." in: The Journal of biological chemistry, Vol. 284, Issue 48, pp. 33233-41, (2009) (PubMed).

Douglas, Lim, Porter, West, Pink, Ge, Wylie, Tibbits, Biggs, Curtis, Palombella, Adams, Fritz, Normant: "The antiproliferative activity of the heat shock protein 90 inhibitor IPI-504 is not dependent on NAD(P)H:quinone oxidoreductase 1 activity in vivo." in: Molecular cancer therapeutics, Vol. 8, Issue 12, pp. 3369-78, (2009) (PubMed).

Target Details for NQO1

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Alternative Name: NQO1 (NQO1 Products)

Background:

Description: This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Aliases: DTD, QR1, DHQU, DIA4, NMOR1, NMORI

Molecular Weight: 31kDa

Gene ID: 1728

HGNC: 1728

UniProt: P15559