NPT antibody
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- Target
- NPT
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB)
- Purification
- purified
- Immunogen
- Purified recombinant fragment of NPT expressed in E. coli.
- Clone
- 4B4D1
- Isotype
- IgG2a
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- Application Notes
- ELISA: 1:10000, WB: 1:500 - 1:2000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Ascitic fluid containing 0.03 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- 4°C, -20°C for long term storage
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Reduction of poliovirus 1, bacteriophages, Salmonella montevideo, and Escherichia coli O157:H7 on strawberries by physical and disinfectant washes." in: Journal of food protection, Vol. 66, Issue 2, pp. 188-93, (2003) (PubMed).
: "Transgenic plants and biosafety: science, misconceptions and public perceptions." in: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, (2001) (PubMed).
: "
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Reduction of poliovirus 1, bacteriophages, Salmonella montevideo, and Escherichia coli O157:H7 on strawberries by physical and disinfectant washes." in: Journal of food protection, Vol. 66, Issue 2, pp. 188-93, (2003) (PubMed).
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- Target
- NPT
- Background
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Description: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
Aliases: N/A
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