NME1 antibody

Catalog No. ABIN969317

Product Details anti-NME1 Antibody

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NME1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: NME1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human NME1 expressed in E. Coli.

Clone: 4B2

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-NME1 antibody (ABIN969317)

Treharne, Giles Best, Mehta: "Transglutaminase 2 and nucleoside diphosphate kinase activity are correlated in epithelial membranes and are abnormal in cystic fibrosis." in: FEBS letters, Vol. 583, Issue 17, pp. 2789-92, (2009) (PubMed).

Egistelli, Chichiarelli, Gaucci, Eufemi, Schininà, Giorgi, Lascu, Turano, Giartosio, Cervoni: "IFI16 and NM23 bind to a common DNA fragment both in the P53 and the cMYC gene promoters." in: Journal of cellular biochemistry, Vol. 106, Issue 4, pp. 666-72, (2009) (PubMed).

Lee, Jeong, Kim, Song, Kang, Lee: "Multiple functions of Nm23-H1 are regulated by oxido-reduction system." in: PLoS ONE, Vol. 4, Issue 11, pp. e7949, (2009) (PubMed).

Target Details for NME1

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Alternative Name: NME1 (NME1 Products)

Background:

Description: non-metastatic cells 1,protein,with a nm23 nucleoside diphosphate kinase gene family,involved in the phosphorylation of nucleoside diphosphates,with a reduced expression in tumor progression to the metastatic phenotype,mutated in agressive neuroblastoma,expressed in lung carcinoma cell lines not in normal lung,pyrimidine biosynthetic pathway. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. Has tumor metastasis-suppressive capacity.Tissue specificity: Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.

Aliases: NB, AWD, NBS, GAAD, NM23, NDPKA, NDPK-A, NM23-H1, NME1

Molecular Weight: 17kDa

Gene ID: 4830

HGNC: 4830

UniProt: P15531

Pathways: Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process