MLH1 antibody (AA 381-483)

Catalog No. ABIN969285

Product Details anti-MLH1 Antibody

Target: MLH1 (MutL Homolog 1 (MLH1))

Binding Specificity: AA 381-483

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: MLH1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.

Clone: 4C9C7

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-MLH1 antibody (ABIN969285)

Geary, Sasieni, Houlston, Izatt, Eeles, Payne, Fisher, Hodgson: "Gene-related cancer spectrum in families with hereditary non-polyposis colorectal cancer (HNPCC)." in: Familial cancer, Vol. 7, Issue 2, pp. 163-72, (2008) (PubMed).

Latta, Mohan, Issekutz: "CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis." in: Immunology, Vol. 121, Issue 4, pp. 555-64, (2007) (PubMed).

Zeng, Kinsella: "A novel role for DNA mismatch repair and the autophagic processing of chemotherapy drugs in human tumor cells." in: Autophagy, Vol. 3, Issue 4, pp. 368-70, (2007) (PubMed).

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1 (MLH1 Products)

Synonyms: CG11482 antibody, Dmel\\CG11482 antibody, dmlh-1 antibody, dmlh1 antibody, zgc:66301 antibody, MLH1 antibody, LOC100232198 antibody, 1110035C23Rik antibody, AI317206 antibody, AI325952 antibody, AI561766 antibody, COCA2 antibody, FCC2 antibody, HNPCC antibody, HNPCC2 antibody, hMLH1 antibody, mutL homolog 1 antibody, CG11482 gene product from transcript CG11482-RB antibody, mutL homolog 1 S homeolog antibody, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody, DNA mismatch repair protein Mlh1 antibody, MLH1 antibody, Mlh1 antibody, mlh1.S antibody, mlh1 antibody, LOC588545 antibody

Background:

Description: DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.

Aliases: FCC2, COCA2, HNPCC

Molecular Weight: 85kDa

Gene ID: 4292

HGNC: 4292

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response