FAK antibody

Catalog No. ABIN969130

Product Details anti-FAK Antibody

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: FAK Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human FAK expressed in E. Coli.

Clone: 10H7

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-FAK antibody (ABIN969130)

Toutant, Costa, Studler, Kadaré, Carnaud, Girault: "Alternative splicing controls the mechanisms of FAK autophosphorylation." in: Molecular and cellular biology, Vol. 22, Issue 22, pp. 7731-43, (2002) (PubMed).

Huang, Cheung, Parsons, Bryer-Ash: "Focal adhesion kinase (FAK) regulates insulin-stimulated glycogen synthesis in hepatocytes." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18151-60, (2002) (PubMed).

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: FAK (PTK2 Products)

Background:

Description: This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. Tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.RD: Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a

Aliases: FAK, FADK, FAK1, FRNK, pp125FAK, PTK2

Molecular Weight: 119kDa

Gene ID: 5747

HGNC: 5747

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling