CDC27 antibody

Catalog No. ABIN969035

Product Details anti-CDC27 Antibody

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC27 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: CDC27 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CDC27 expressed in E. Coli.

Clone: 5C12

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-CDC27 antibody (ABIN969035)

Goto, Eddy: "Speriolin is a novel spermatogenic cell-specific centrosomal protein associated with the seventh WD motif of Cdc20." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 42128-38, (2004) (PubMed).

Koloteva-Levine, Pinchasi, Pereman, Zur, Brandeis, Elroy-Stein: "The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3577-87, (2004) (PubMed).

Target Details for CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Alternative Name: CDC27 (CDC27 Products)

Background:

Description: Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Aliases: APC3, HNUC, ANAPC3, CDC27Hs, D0S1430E, D17S978E,

Molecular Weight: 91kDa

Gene ID: 996

HGNC: 996

UniProt: P30260