CD3 epsilon antibody

Catalog No. ABIN969024

Product Details anti-CD3 epsilon Antibody

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Purpose: CD3E Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of CD3E expressed in E. Coli.

Clone: 4E2

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-CD3 epsilon antibody (ABIN969024)

Boskovic, Arnold, Stilion, Gordon, Sim, Rivera-Calzada, Wienke, Isacke, Martinez-Pomares, Llorca: "Structural model for the mannose receptor family uncovered by electron microscopy of Endo180 and the mannose receptor." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8780-7, (2006) (PubMed).

Urano, Iiduka, Sugiyama, Akiyama, Uzawa, Matsumoto, Kawasaki, Tashiro: "Involvement of the mouse Prp19 gene in neuronal/astroglial cell fate decisions." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7498-514, (2006) (PubMed).

Xu, Call, Wucherpfennig: "A membrane-proximal tetracysteine motif contributes to assembly of CD3deltaepsilon and CD3gammaepsilon dimers with the T cell receptor." in: The Journal of biological chemistry, Vol. 281, Issue 48, pp. 36977-84, (2006) (PubMed).

Li: "Therapeutic biology: checkpoint pathway activation therapy, HIV Tat, and transkingdom RNA interference." in: Journal of cellular physiology, Vol. 209, Issue 3, pp. 695-700, (2006) (PubMed).

Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, (2005) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E (CD3E Products)

Background:

Description: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

Aliases: T3E, TCRE, FLJ18683, CD3E

Molecular Weight: 23kDa

Gene ID: 916

HGNC: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway