BID antibody

Catalog No. ABIN968979

Product Details anti-BID Antibody

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: BID Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant full length protein of human BID expressed in E. Coli

Clone: 3C5

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-BID antibody (ABIN968979)

Wan, Liu, Xing, Chen: "Bid is required in NPe6-PDT-induced apoptosis." in: Photochemistry and photobiology, Vol. 84, Issue 1, pp. 250-7, (2008) (PubMed).

Wu, Xing, Chen, Wang: "Bid is not required for Bax translocation during UV-induced apoptosis." in: Cellular signalling, Vol. 19, Issue 12, pp. 2468-78, (2007) (PubMed).

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID (BID Products)

Synonyms: FP497 antibody, 2700049M22Rik antibody, AI875481 antibody, AU022477 antibody, BID antibody, bid antibody, si:ch211-238n5.6 antibody, fp497 antibody, xbid antibody, DKFZp469E066 antibody, BH3 interacting domain death agonist antibody, BH3 interacting domain death agonist S homeolog antibody, BID antibody, Bid antibody, bida antibody, bid.S antibody, bid antibody

Background:

Description: This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.Tissue specificity: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level)

Aliases: FP497, MGC15319, MGC42355, BID

Molecular Weight: 22kDa

Gene ID: 637

HGNC: 637

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity