TCR alpha/beta antibody

Catalog No. ABIN968932

Product Details anti-TCR alpha/beta Antibody

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Reactivity: Rat, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR alpha/beta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Cross-Reactivity (Details): Non-Human Primates, Rat

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Rat T blasts and erythrocytes

Clone: R73

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TCR alpha/beta antibody (ABIN968932)

Fuller, Bogdani, Tupling, Jensen, Pefley, Manavi, Cort, Blankenhorn, Mordes, Lernmark, Kwitek: "Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene." in: Physiological genomics, Vol. 38, Issue 1, pp. 89-97, (2009) (PubMed).

Müller, van den Brandt, Odoardi, Tischner, Herath, Flügel, Reichardt: "A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats." in: The Journal of clinical investigation, Vol. 118, Issue 4, pp. 1405-16, (2008) (PubMed).

Radojevic, Arsenovic-Ranin, Kosec, Pesic, Pilipovic, Perisic, Plecas-Solarovic, Leposavic: "Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level." in: The Journal of endocrinology, Vol. 192, Issue 3, pp. 669-82, (2007) (PubMed).

Shao, Sun, Kaplan, Sun: "Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal-binding protein 1177-1191." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 4, pp. 2849-54, (2004) (PubMed).

Kerstan, Hünig: "Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 3, pp. 1341-5, (2004) (PubMed).

Li, Thanikachalam, Pang, Kawaharada, Aitouche, Pham: "A clinically relevant CTLA4-Ig-based regimen induces chimerism and tolerance to heart grafts." in: The Annals of thoracic surgery, Vol. 72, Issue 4, pp. 1306-10, (2001) (PubMed).

Carlson, Jansson, Larsson, Bucht, Lorentzen: "The endogenous adjuvant squalene can induce a chronic T-cell-mediated arthritis in rats." in: The American journal of pathology, Vol. 156, Issue 6, pp. 2057-65, (2000) (PubMed).

Kraus, Lambracht, Wonigeit, Hünig: "Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition." in: European journal of immunology, Vol. 26, Issue 11, pp. 2582-6, (1997) (PubMed).

Target Details for TCR alpha/beta

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Alternative Name: TCR alpha/beta (TCR alpha/beta Products)

Background: The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.,TCRA/B