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LIMS1 antibody (AA 120-220)

LIMS1 Reactivity: Human, Mouse, Rat, Dog, Chicken WB, IF Host: Mouse Monoclonal 49-PINCH unconjugated
Catalog No. ABIN968921
  • Target See all LIMS1 Antibodies
    LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))
    Binding Specificity
    • 15
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 120-220
    Reactivity
    • 58
    • 24
    • 16
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Human, Mouse, Rat, Dog, Chicken
    Host
    • 48
    • 10
    Mouse
    Clonality
    • 48
    • 10
    Monoclonal
    Conjugate
    • 19
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LIMS1 antibody is un-conjugated
    Application
    • 18
    • 13
    • 13
    • 13
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Rat (Rattus), Mouse (Murine)
    No Cross-Reactivity
    Dog (Canine), Chicken
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human PINCH aa. 120-220
    Clone
    49-PINCH
    Isotype
    IgG2a
    Top Product
    Discover our top product LIMS1 Primary Antibody
  • Comment

    Related Products: ABIN968586, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Campana, Myers, Rearden: "Identification of PINCH in Schwann cells and DRG neurons: shuttling and signaling after nerve injury." in: Glia, Vol. 41, Issue 3, pp. 213-23, (2003) (PubMed).

    Tu, Li, Goicoechea, Wu: "The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2425-34, (1999) (PubMed).

    Tu, Li, Wu: "Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways." in: Molecular biology of the cell, Vol. 9, Issue 12, pp. 3367-82, (1999) (PubMed).

  • Target
    LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))
    Alternative Name
    PINCH (LIMS1 Products)
    Synonyms
    PINCH antibody, PINCH-1 antibody, PINCH1 antibody, Pinch1 antibody, RGD1560732 antibody, LIMS3 antibody, LIMS3L antibody, 2310016J22Rik antibody, 4921524A02Rik antibody, AI507642 antibody, AU021743 antibody, AW551584 antibody, C430041B13Rik antibody, Lims1l antibody, LIM zinc finger domain containing 1 antibody, LIM and senescent cell antigen-like domains 1 antibody, LIMS1 antibody, Lims1 antibody
    Background
    Integrins are transmembrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are heterodimers composed of alpha and beta subunits, which interact with extracellular matrix and cytoskeletal proteins. Signal transduction through intergin receptors may be regulated by integrin-linked kinase (ILK). ILK is a widely expressed Ser/Thr protein kinase that contains four ankyrin-like repeats in the N-terminal region, a phoshoinositide lipid-binding motif at amino acids 180-212, and an integrin binding site at amino acids 293-451. The ankyrin repeats interact with a LIM domain-only protein called PINCH that also binds the adaptor protein, Nck-2. This interaction implicates ILK in growth factor receptor pathways. The PINCH adaptor protein also contains a leucine-rich nuclear export signal and a nuclear localization signal. These two motifs implicate PINCH in nuclear functions, and in Schwann cells PINCH has been found in the nucleus, as well as, cytoplasm and perinuclear regions. Thus, PINCH is an adaptor protein that functions in both integrin signaling and nuclear functions.
    Molecular Weight
    37 kDa
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