Actin antibody

Catalog No. ABIN968903

Product Details anti-Actin Antibody

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Chicken gizzard muscle Actin

Clone: C4-actin

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Actin antibody (ABIN968903)

Yamamori, Itakura, Sugaya, Katsumata, Sakagami, Takahashi: "Differential expression of SNAP-25 family proteins in the mouse brain." in: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, (2011) (PubMed).

Pantaloni, Le Clainche, Carlier: "Mechanism of actin-based motility." in: Science (New York, N.Y.), Vol. 292, Issue 5521, pp. 1502-6, (2001) (PubMed).

Mitchison, Cramer: "Actin-based cell motility and cell locomotion." in: Cell, Vol. 84, Issue 3, pp. 371-9, (1996) (PubMed).

Hanstein, Lange, Schneider-Poetsch, Grolig, Wagner: "Detection of actin and localization of phytochrome in the green alga Mougeotia by monoclonal antibodies." in: Acta histochemica. Supplementband, Vol. 41, Issue 9, pp. 223-30, (1992) (PubMed).

Target Details for Actin

Target: Actin

Abstract: Actin Products

Synonyms: Act57 antibody, Act57A antibody, Act57b antibody, Actin antibody, BEST:RE38067 antibody, C antibody, CG10067 antibody, CG30294 antibody, Dmel\\CG10067 antibody, M antibody, T10 antibody, act 57A antibody, act57A antibody, act57B antibody, act57b antibody, actin antibody, anon-EST:CL2c12 antibody, 143060_f_at antibody, CG7478 antibody, D antibody, Dmel\\CG7478 antibody, act 79B antibody, act79B antibody, mus79B antibody, Act(88F) antibody, Act88-F antibody, Act88f antibody, CG5178 antibody, Dmel\\CG5178 antibody, E antibody, Ifm(3)7 antibody, act 88F antibody, act88F antibody, rsd antibody, Act87e antibody, CG18290 antibody, Dmel\\CG18290 antibody, act 87E antibody, act87E antibody, mus87E antibody, Act88F antibody, Dsim\\GD19025 antibody, GD19025 antibody, dsim_GLEANR_2829 antibody, 42A antibody, A antibody, AFFX-Dros-ACTIN_M_r_at antibody, Act antibody, Act42 antibody, Act42a antibody, Actin/BAP47 antibody, BAP47 antibody, CG12051 antibody, Dmel\\CG12051 antibody, act 42A antibody, act42A antibody, cytoplasmic antibody, Actin 57B antibody, Actin 79B antibody, Actin 88F antibody, Actin 87E antibody, Actin-like protein C08B11.6 antibody, hypothetical protein antibody, ACT_COLSC Actin (ISS) antibody, Actin 42A antibody, actin gamma 1 antibody, actin antibody, Actin, gamma 1 antibody, si:ch211-241j12.3 antibody, Act57B antibody, Act79B antibody, Act88F antibody, Act87E antibody, arp-6 antibody, Dsim\Act88F antibody, ACTIN antibody, Act42A antibody, ACTG1 antibody, LOC548170 antibody, Bm1_16810 antibody, IDA5 antibody, LOC100342017 antibody, si:ch211-241j12.3 antibody

Background: Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton. This reorganization of actin filaments results from interactions between actin and actin-binding proteins. Actin is a 42-kDa protein that is known as G-actin in its monomeric form. Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin. F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks. The latter are highly concentrated beneath the plasma membrane and form the actin cortex. Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins. These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures. Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins. The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.

Molecular Weight: 42 kDa