RUVBL2 antibody (AA 180-228)

Catalog No. ABIN968851

Product Details anti-RUVBL2 Antibody

Target: RUVBL2 (RuvB-Like 2 (E. Coli) (RUVBL2))

Binding Specificity: AA 180-228

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This RUVBL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Reptin 52/TIF-496 aa. 180-228

Clone: 42-TIP49b

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-RUVBL2 antibody (ABIN968851)

Cho, Bhoumik, Broday, Ivanov, Rosenstein, Ronai: "TIP49b, a regulator of activating transcription factor 2 response to stress and DNA damage." in: Molecular and cellular biology, Vol. 21, Issue 24, pp. 8398-413, (2001) (PubMed).

Bauer, Chauvet, Huber, Usseglio, Rothbächer, Aragnol, Kemler, Pradel: "Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity." in: The EMBO journal, Vol. 19, Issue 22, pp. 6121-30, (2000) (PubMed).

Kanemaki, Kurokawa, Matsu-ura, Makino, Masani, Okazaki, Morishita, Tamura: "TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22437-44, (1999) (PubMed).

Target Details for RUVBL2

Target: RUVBL2 (RuvB-Like 2 (E. Coli) (RUVBL2))

Alternative Name: TIP49b (RUVBL2 Products)

Synonyms: ECP51 antibody, INO80J antibody, REPTIN antibody, RVB2 antibody, TIH2 antibody, TIP48 antibody, TIP49B antibody, mp47 antibody, p47 antibody, reptin antibody, wu:fi25f01 antibody, zreptin antibody, MGC52995 antibody, tip48 antibody, xReptin antibody, rvb2 antibody, ecp51 antibody, cgi-46 antibody, tip49b antibody, MGC69398 antibody, RUVBL2 antibody, AAEL010341 antibody, Reptin antibody, rept antibody, RuvB like AAA ATPase 2 antibody, RuvB-like protein 2 antibody, RuvB-like AAA ATPase 2 antibody, RuvB like AAA ATPase 2 L homeolog antibody, ruvB-like 2 antibody, TATA box-binding protein antibody, ruvb-like 2 antibody, ruvB-like helicase 2 antibody, RUVBL2 antibody, Ruvbl2 antibody, ruvbl2 antibody, ruvbl2.L antibody, LOC726816 antibody, HBUT_RS02095 antibody, HAN_2g314 antibody, LOC100282179 antibody, LOC100284260 antibody, LOC5573243 antibody

Background: Processes involving DNA transcription, replication and repair are necessary in maintaining normal cell function. For each of these processes, the double stranded NA must first be unwound. This is achieved in an ATP-dependent manner by enzymes termed DNA helicases. Reptin52/TIP49b is a DNA helicase that directly interacts with Pontin52/TIP49a. Reptin52/TIP49b and Pontin52/TIP49a belong to a family of highly conserved proteins that share homology with the bacterial protein RuvB, a helicase involved in double-strand break repair and homologous recombination. Both are TATA binding protein (TBP) interacting proteins that bind to beta-Catenin and are among the 12 polypeptides that comprise the INO80 chromatin-remodeling complex. It has also been demonstrated that Reptin52/TIP49b and Pontin52/TIP49a can be found in a complex with the proto- ncogene c-myc. Reptin52/TIP49b and Pontin52/TIP49a have antagonistic roles as Reptin/TIP49b represses gene activation mediated by beta-Catenin and c-myc, whereas Pontin52/TIP49a stimulates gene activation. Thus, Reptin52/TIP49b and Pontin52/TIP49a are transcriptional co-factors that work together to help control multiple pathways.

Molecular Weight: 52 kDa

Pathways: Telomere Maintenance