HERC2 antibody (AA 1781-1974)

Catalog No. ABIN968838

Product Details anti-HERC2 Antibody

Target: HERC2 (Hect Domain and RLD 2 (HERC2))

Binding Specificity: AA 1781-1974

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HERC2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse HERC2 aa. 1781-1974

Clone: 17-HERC2

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-HERC2 antibody (ABIN968838)

Ji, Rebert, Joslin, Higgins, Schultz, Nicholls: "Structure of the highly conserved HERC2 gene and of multiple partially duplicated paralogs in human." in: Genome research, Vol. 10, Issue 3, pp. 319-29, (2000) (PubMed).

Lehman, Nakatsu, Ching, Bronson, Oakey, Keiper-Hrynko, Finger, Durham-Pierre, Horton, Newton, Lyon, Brilliant: "A very large protein with diverse functional motifs is deficient in rjs (runty, jerky, sterile) mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 16, pp. 9436-41, (1998) (PubMed).

Target Details for HERC2

Target: HERC2 (Hect Domain and RLD 2 (HERC2))

Alternative Name: HERC2 (HERC2 Products)

Synonyms: CG11734 antibody, Dmel\\CG11734 antibody, D15F32S1h antibody, D7H15F32S1 antibody, D7H15F37S1 antibody, jdf2 antibody, mKIAA0393 antibody, rjs antibody, D15F37S1 antibody, SHEP1 antibody, p528 antibody, HECT and RLD domain containing protein 2 antibody, HECT and RLD domain containing E3 ubiquitin protein ligase 2 antibody, HERC2 antibody, Herc2 antibody

Background: Prader-Willi and Angelman syndromes are genetic disorders that result from recombination between chromsome-specific low-copy repeats (duplicons). ERC2(rjs) is the transcript found in the duplicon for these disorders, and recessive mutation of HERC2 leads to a developmental syndrome in mice, referred to as runty jerky sterile (rjs) and juvenile development and fertility 2 (jdf2). HERC2 is homologous to the HERC1 and HERC3 HECT-domain containing proteins. The sequence of HERC2 includes three RCC1-like domains (RLD), a C-terminal ECT domain, and a ZZ-type zinc finger. The RCC1 repeats are similar to those found in cytochrome b5 and the HECT domain is found in E6-AP ubiquitin ligase. HERC2 may function as both a guanine nucleotide exchange factor and E3 biquitin ligase based on its conserved motifs and observation from mouse mutation studies. HERC2 mRNA is expressed highest in brain and testes, but is also found in heart, lung, liver, skeletal muscle, and kidney. Thus, HERC2 may function in protein trafficking and degradation pathways in various tissues.

Molecular Weight: 527 kDa