GGA3 antibody (AA 424-542)
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- Target See all GGA3 Antibodies
- GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))
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Binding Specificity
- AA 424-542
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GGA3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Characteristics
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human GGA3 aa. 424-542
- Clone
- 8-GGA3
- Isotype
- IgG1
- Top Product
- Discover our top product GGA3 Primary Antibody
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- Comment
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Related Products: ABIN968537, ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store undiluted at -20° C.
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A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi." in: Molecular biology of the cell, Vol. 11, Issue 4, pp. 1241-55, (2000) (PubMed).
: "GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 81-94, (2000) (PubMed).
: "A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 67-80, (2000) (PubMed).
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A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi." in: Molecular biology of the cell, Vol. 11, Issue 4, pp. 1241-55, (2000) (PubMed).
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- Target
- GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))
- Alternative Name
- GGA3 (GGA3 Products)
- Synonyms
- C230037M19Rik antibody, mKIAA0154 antibody, golgi associated, gamma adaptin ear containing, ARF binding protein 3 antibody, Gga3 antibody, GGA3 antibody
- Background
- The ADP-ribosylation factors (ARFs) are a family of small GTPases in the ARF superfamily that include ARFs and ARF-like (ARLs) proteins. At least six ARFs have been identified in humans: ARF1, ARF2, ARF3, ARF4, ARF5, and ARF6. ARFs are involved in intravesicular acidification and fusion of microsomal vesicles, endosome fusion, nuclear membrane assembly, and formation of clathrin-coated vesicles. GGAs are ARF-binding proteins that act as adaptor coat proteins associated with the Golgi complex. GGA1, GGA2, and GGA3 are homologous proteins that contain N-terminal VHS domains, a GGA and TOM homology region (GAT), and a C-terminal region homologous to the ear domain of gamma-adaptins. GGAs co-localize with Golgi markers in the TGN, and GGA3 is found present in coated vesicles and buds associated with the TGN. The GAT domain of GGA3 facilitates ARF1 binding, Golgi localization, and dissociation from ARF-regulated membranes. The C-terminal region of GGAs bind to MAP1A and rabaptin-5, which are binding partners of gamma-adaptins. Overexpression of GGAs alters the distribution of markers normally found in the TGN. Thus, GGAs are ARF binding proteins that regulate vesicle dynamics in the TGN.
- Molecular Weight
- 90 kDa
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