CDK1 antibody (pTyr15)

Catalog No. ABIN968812

Product Details anti-CDK1 Antibody

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Binding Specificity: pTyr15

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity (Details): Predicted: Mouse, Rat

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Cdc2 (pY15) Peptide

Clone: 44-Cdk1-Cdc2

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN967704

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-CDK1 antibody (ABIN968812)

Borgne, Meijer: "Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition." in: The Journal of biological chemistry, Vol. 271, Issue 44, pp. 27847-54, (1996) (PubMed).

Uckun, Tuel-Ahlgren, Waddick, Jun, Jin, Myers, Rowley, Burkhardt, Bolen: "Physical and functional interactions between Lyn and p34cdc2 kinases in irradiated human B-cell precursors." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6389-97, (1996) (PubMed).

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: Cdk1/Cdc2 (CDK1 Products)

Synonyms: CDC2 antibody, CDC28A antibody, P34CDC2 antibody, Cdc2 antibody, Cdc2a antibody, p34 antibody, cdc2 antibody, wu:fc30e01 antibody, zgc:92032 antibody, PSTAIR antibody, cdc-2 antibody, cdc2-a antibody, cdc28a antibody, cdc2x1.1 antibody, p34cdc2 antibody, xcdc2 antibody, 5363 antibody, CDCDm antibody, CDK1 antibody, CDK1/CDC2 antibody, CG5363 antibody, Cdk-1 antibody, Cdk1 antibody, Dcdc2 antibody, Dm cdc2 antibody, DmCdc2 antibody, DmCdk1 antibody, Dmcdc2 antibody, Dmel\\CG5363 antibody, cdc antibody, cdc2Dm antibody, cdk1 antibody, dCdk1 antibody, group 4 antibody, l(2)31Eh antibody, CDC2A antibody, CDC2AAT antibody, CDK2 antibody, CDKA1 antibody, CDKA;1 antibody, CYCLIN-DEPENDENT KINASE A;1 antibody, cell division control 2 antibody, cdc2-b antibody, cdc2a antibody, cdc2x1.2 antibody, POL3 antibody, pold antibody, cyclin dependent kinase 1 antibody, cyclin dependent kinase like 1 antibody, Cell division control protein 2 1 antibody, cyclin-dependent kinase 1 antibody, cyclin-dependent kinase 1 S homeolog antibody, Cyclin-dependent kinase 1 antibody, cell division control 2 antibody, cell division control protein2 homolog antibody, cyclin-dependent kinase 1 L homeolog antibody, polymerase (DNA directed), delta 1, catalytic subunit L homeolog antibody, cyclin-dependent protein kinase Cdk1/Cdc2 antibody, CDK1 antibody, CDKL1 antibody, POPTR_0004s14080g antibody, Cdk1 antibody, cdk1 antibody, cdk1.S antibody, CDC2 antibody, cdc2 antibody, cdk-1 antibody, cdk1.L antibody, pold1.L antibody

Background: Progression of the mammalian cell cycle is regulated by phosphorylation of many key proteins. Several classes of cyclins (A-E) act as regulatory subunits for cyclin-dependent kinases (cdks). Cdk1/Cdc2 (p34 [cdc2]) is the catalytic subunit of the maturation promoting factor (MPF), which includes the regulatory subunit cyclin B. During late S and G2 phase, cyclin B synthesis increases, allowing it to bind Cdc2. This begins the transition into M-phase of the mammalian cell cycle by initiating a series of phosphorylation and dephosphorylation events that lead to activation of the Cdc2/cyclin B complex. After binding to cyclin B, cdc2 is phosphorylated on Thr-14, by Myt1, and Tyr-15, by wee1 or mik1, yielding an inactive pre-MPF complex during G2 phase. Phosphorylation of cdc2 on Thr-161 is performed by a cdk7/cyclin H complex and is necessary for activation of the cdc2 complex. Dephosphorylation of Thr-14 and Tyr-15 by CDC25 occurs at the end of G2 phase and completes activation of the cdc2/Cyclin B complex and facilitates entry into mitosis. During mitosis, cyclin B is targeted for degradation and Cdc2 becomes inactive again.
Synonyms: p34 [cdc2]

Molecular Weight: 34 kDa

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA