ASAH1 antibody (AA 88-182)

Catalog No. ABIN968809

Product Details anti-ASAH1 Antibody

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Binding Specificity: AA 88-182

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASAH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Acid Ceramidase aa. 88-182

Clone: 23-Acid Ceramidase

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-ASAH1 antibody (ABIN968809)

Ferlinz, Kopal, Bernardo, Linke, Bar, Breiden, Neumann, Lang, Schuchman, Sandhoff: "Human acid ceramidase: processing, glycosylation, and lysosomal targeting." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 35352-60, (2001) (PubMed).

Strelow, Bernardo, Adam-Klages, Linke, Sandhoff, Krönke, Adam: "Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death." in: The Journal of experimental medicine, Vol. 192, Issue 5, pp. 601-12, (2000) (PubMed).

Bernardo, Hurwitz, Zenk, Desnick, Ferlinz, Schuchman, Sandhoff: "Purification, characterization, and biosynthesis of human acid ceramidase." in: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11098-102, (1995) (PubMed).

Target Details for ASAH1

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Alternative Name: Acid Ceramidase (ASAH1 Products)

Synonyms: AC antibody, ACDase antibody, ASAH antibody, PHP antibody, PHP32 antibody, SMAPME antibody, 2310081N20Rik antibody, Asah antibody, MGC82286 antibody, asah1 antibody, zgc:101637 antibody, ASAH1 antibody, zgc:66026 antibody, N-acylsphingosine amidohydrolase 1 antibody, N-acylsphingosine amidohydrolase (acid ceramidase) 1 antibody, N-acylsphingosine amidohydrolase (acid ceramidase) 1 L homeolog antibody, N-acylsphingosine amidohydrolase (acid ceramidase) 1a antibody, Acid ceramidase antibody, N-acylsphingosine amidohydrolase (acid ceramidase) 1b antibody, ASAH1 antibody, Asah1 antibody, asah1.L antibody, asah1a antibody, asah-1 antibody, asah1b antibody

Background: Ceramide is a sphingolipid that exhibits a wide variety of functions, including monocyte differentiation, apoptosis, neurite outgrowth, and Ca2+ transport. It also serves as the precursor of many sphingolipids and anchors these into the outer leaflet of the plasma membrane via hydrophobic interactions. Acid ceramidase is a lysosomal enzyme that was purified from human urine. It is synthesized as a 55kDa precursor protein, which is then processesed into the mature alpha-subunit (13kDa) and beta-subunit (40kDa). Acid ceramidase catalyzes the hydrolysis of ceramide into free fatty acid and sphingosine. Sphingosine, and its phosphorylated form, sphingosine-1-phosphate (SPP), have been shown to inhibit PKC activity and act as a second messenger in cell proliferation and differentiation. Acid ceramidase is also the cause of a lysosomal storage disorder known as Farber’s disease. This disease is characterized by an accumulation of ceramide in tissues, leading to swelling and pain of the joints and extremities, pulmonary insufficiency, and death at an early age. Thus, acid ceramidase is necessary in the hydrolysis of ceramide and is the cause of Farber’s disease.

Molecular Weight: 13 kDa