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Nicastrin antibody (AA 168-289)

NCSTN Reactivity: Human, Mouse, Rat, Chicken, Dog WB, IF Host: Mouse Monoclonal 35-Nicastrin unconjugated
Catalog No. ABIN968805
  • Target See all Nicastrin (NCSTN) Antibodies
    Nicastrin (NCSTN)
    Binding Specificity
    • 15
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 168-289
    Reactivity
    • 36
    • 32
    • 21
    • 5
    • 5
    • 4
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Dog
    Host
    • 50
    • 2
    Mouse
    Clonality
    • 43
    • 9
    Monoclonal
    Conjugate
    • 20
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Nicastrin antibody is un-conjugated
    Application
    • 38
    • 13
    • 13
    • 9
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Nicastrin aa. 168-289
    Clone
    35-Nicastrin
    Isotype
    IgG2a
    Top Product
    Discover our top product NCSTN Primary Antibody
  • Comment

    Related Products: ABIN967389, ABIN968553

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Chen, Yu, Arawaka, Nishimura, Kawarai, Yu, Tandon, Supala, Song, Rogaeva, Milman, Sato, Yu, Janus, Lee, Song, Zhang, Fraser, St George-Hyslop: "Nicastrin binds to membrane-tethered Notch." in: Nature cell biology, Vol. 3, Issue 8, pp. 751-4, (2001) (PubMed).

    Yu, Nishimura, Arawaka, Levitan, Zhang, Tandon, Song, Rogaeva, Chen, Kawarai, Supala, Levesque, Yu, Yang, Holmes, Milman, Liang, Zhang, Xu, Sato, Rogaev, Smith, Janus, Zhang, Aebersold, Farrer, Sorbi et al.: "Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. ..." in: Nature, Vol. 407, Issue 6800, pp. 48-54, (2000) (PubMed).

  • Target
    Nicastrin (NCSTN)
    Alternative Name
    Nicastrin (NCSTN Products)
    Synonyms
    CG7012 antibody, Dmel\\CG7012 antibody, Nct antibody, Nic antibody, S5A antibody, ST96B antibody, agro antibody, dNct antibody, nic antibody, NCSTN antibody, DKFZp469O2418 antibody, APH2 antibody, 9430068N19Rik antibody, AA727311 antibody, Aph2 antibody, D1Dau13e antibody, Kiaa0253 antibody, mKIAA0253 antibody, nicastrin antibody, Nicastrin antibody, NCSTN antibody, nct antibody, ncstn antibody, CpipJ_CPIJ001617 antibody, Ncstn antibody, aph-2 antibody
    Background
    Amyloid precursor protein (APP) gene encodes multiple APPs ranging from 695 to 770 amino acids. The unprocessed form of APP is a putative cell surface receptor that possesses neurite-promoting activity, and is involved in synaptic vesicle recycling. Processing of APP by sequential enzymatic activity of beta- and gamma-secretase, and the presenilin proteins PS1 and PS2, produces APP fragments that may have unique functions. beta-secretase activity cleaves the extracellular portion of APP leading to asecreted APP form, while gamma-secretase activity produces beta-Amyloid peptide (39-43 amino acids). The Abeta peptide produces abnormal plaques in the cerebral cortex and blood vesselwalls during Alzheimer's disease. Nicastrin is a PS1 associated transmembrane protein,that contains N-terminal glycosylation and myristoylation sites. Nicastrin can bind full length APP and the fragments produced by gamma-secretase. In C. elegans, suppression of nicastrin expression produces phenotypes that mimic those produced when notch signaling proteins are suppressed. In Drosophila, deficiencies in nicastrin prevent cleavage of the intracellular portion of Notch. Thus, nicastrin may be a functional component of presenilins and gamma-secretase complexes, which process Notch and APP transmembrane receptors.
    Molecular Weight
    110 kDa
    Pathways
    Notch Signaling, Neurotrophin Signaling Pathway
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