Coilin antibody (AA 226-332)

Catalog No. ABIN968730

Product Details anti-Coilin Antibody

Target: Coilin (COIL)

Binding Specificity: AA 226-332

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Coilin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Coilin aa. 226-332

Clone: 56-Coilin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968586, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Coilin antibody (ABIN968730)

Bellini, Gall: "Coilin shuttles between the nucleus and cytoplasm in Xenopus oocytes." in: Molecular biology of the cell, Vol. 10, Issue 10, pp. 3425-34, (1999) (PubMed).

Andrade, Chan, Raska, Peebles, Roos, Tan: "Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin." in: The Journal of experimental medicine, Vol. 173, Issue 6, pp. 1407-19, (1991) (PubMed).

Target Details for Coilin

Target: Coilin (COIL)

Alternative Name: Coilin (COIL Products)

Synonyms: p80c antibody, coil antibody, CG8710 antibody, Coilin antibody, Dmel\\CG8710 antibody, LOC100218802 antibody, F3F19.5 antibody, F3F19_5 antibody, CLN80 antibody, p80-coilin antibody, C79982 antibody, Cln80 antibody, p80 antibody, wu:fb08h11 antibody, sph1 antibody, Coilin antibody, coilin antibody, sphere organelles protein-like protein antibody, coilin p80 antibody, coilin L homeolog antibody, p80c antibody, COIL antibody, coil antibody, AT1G13030 antibody, Coil antibody, coil.L antibody

Background: Ramon Cajal described small silver-staining organelles in the nuclei of pyramidal neurons, which have been referred to as cajal bodies or coiled bodies. An 80 kDa protein called p80 coilin was identified through its co-localization to coiled bodies. Coilin is a molecular marker of coiled bodies, but can also be found in the nucleoplasm, and may shuttle back and forth between the cytoplasm and nucleoplasm. Studies of GFP-coilin show that coiled bodies can move within the nucleoplasm to and from nucleoli. Other proteins that have been co-localized with coilin to the coiled bodies include cell cycle proteins, snRNPs, U3 snRNA, U7 snRNA, and several nucleolar proteins. Coiled bodies may recruit U7 snRNP and the stem-loop-binding protein to the chromosomal sites of histone gene transcription. Possibly, coilin is important for the formation of coiled bodies that act as sites for preassembly of transcriptosomes, which facilitate gene transcription and RNA processing.

Molecular Weight: 80 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization