TOPBP1 antibody (AA 204-416)

Catalog No. ABIN968690

Product Details anti-TOPBP1 Antibody

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Binding Specificity: AA 204-416

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOPBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TopBP1 aa. 204-416

Clone: 33-TopBP1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TOPBP1 antibody (ABIN968690)

Yamane, Katayama, Tsuruo: "The BRCT regions of tumor suppressor BRCA1 and of XRCC1 show DNA end binding activity with a multimerizing feature." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 678-84, (2001) (PubMed).

Yamane, Tsuruo: "Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA." in: Oncogene, Vol. 18, Issue 37, pp. 5194-203, (1999) (PubMed).

Yamane, Kawabata, Tsuruo: "A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator." in: European journal of biochemistry / FEBS, Vol. 250, Issue 3, pp. 794-9, (1998) (PubMed).

Target Details for TOPBP1

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Alternative Name: TopBP1 (TOPBP1 Products)

Synonyms: RGD1562949 antibody, TOPBP1 antibody, cut5 antibody, F2P24.3 antibody, F2P24_3 antibody, meiosis defective 1 antibody, TOP2BP1 antibody, 1110031N14Rik antibody, 2810429C13Rik antibody, AI256758 antibody, D430026L04Rik antibody, mKIAA0259 antibody, TopBP1-A antibody, TopBP1-B antibody, Xmus101 antibody, XtopBP antibody, mus101 antibody, top2bp1 antibody, DNA topoisomerase II binding protein 1 antibody, topoisomerase (DNA) II binding protein 1 antibody, dna topoisomerase ii binding protein 1 (IC) antibody, transcription coactivator antibody, DNA topoisomerase II binding protein 1 L homeolog antibody, Topbp1 antibody, topbp1 antibody, TOPBP1 antibody, MEI1 antibody, topbp1.L antibody

Background: Eukaryotic DNA topoisomerase II, a ubiquitous ATP-dependent type II topoisomerase, is an essential nuclear enzyme in DNA replication and transcription, chromatin segregation, and cell cycle progression. Topoisomerases transiently break a pair of complementary strands in double-stranded DNA to form a gate for the passage of duplex DNA. Two isoforms of DNA topoisomerase II have been identified: topo IIalpha and topo IIbeta. These exhibit a high degree of homology, except for some divergence in the C-terminal region. Both contain multiple bipartite nuclear localization sequences (NLS) that mediate their subnuclear localization. DNA Topoisomerase II Binding Protein 1 (TopBP1) binds the C-terminal region of Topo IIbeta via its N-terminal region. In addition, TopBP1 contains a putative ADP-ribosylation site, two N-terminal NLS domains, and 8 repeating BRCA1 C-terminal (BRCT) domains found in DNA repair proteins, such as BRCA1, XRCC1, and Rad4. The BRCT domains of TopBP1 have been shown to bind DNA breaks, DNA nicks, and DNA termini, but not circular intact DNA. Thus, TopBP1 may localize topoisomerases to sites of DNA damage.
Synonyms: Topoisomerase II Binding Protein 1

Molecular Weight: 161 kDa

Pathways: Chromatin Binding