XPO1 antibody (AA 2-122)

Catalog No. ABIN968674

Product Details anti-XPO1 Antibody

Target: XPO1 (Exportin 1 (XPO1))

Binding Specificity: AA 2-122

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This XPO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human hCRM1 aa. 2-122

Clone: 17-Exportin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968553, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-XPO1 antibody (ABIN968674)

Kamura, Hara, Matsumoto, Ishida, Okumura, Hatakeyama, Yoshida, Nakayama, Nakayama: "Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase." in: Nature cell biology, Vol. 6, Issue 12, pp. 1229-35, (2004) (PubMed).

Ishida, Hara, Kamura, Yoshida, Nakayama, Nakayama: "Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 14355-8, (2002) (PubMed).

Massenet, Pellizzoni, Paushkin, Mattaj, Dreyfuss: "The SMN complex is associated with snRNPs throughout their cytoplasmic assembly pathway." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6533-41, (2002) (PubMed).

Paraskeva, Izaurralde, Bischoff, Huber, Kutay, Hartmann, Lührmann, Görlich: "CRM1-mediated recycling of snurportin 1 to the cytoplasm." in: The Journal of cell biology, Vol. 145, Issue 2, pp. 255-64, (1999) (PubMed).

Boer, Bonten-Surtel, Grosveld: "Overexpression of the nucleoporin CAN/NUP214 induces growth arrest, nucleocytoplasmic transport defects, and apoptosis." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1236-47, (1998) (PubMed).

Fornerod, Ohno, Yoshida, Mattaj: "CRM1 is an export receptor for leucine-rich nuclear export signals." in: Cell, Vol. 90, Issue 6, pp. 1051-60, (1997) (PubMed).

Target Details for XPO1

Target: XPO1 (Exportin 1 (XPO1))

Alternative Name: Exportin-1 (XPO1 Products)

Synonyms: CG13387 antibody, CRM1 antibody, Crm1 antibody, DCRM1 antibody, Dmel\\CG13387 antibody, Emb antibody, XPO-1 antibody, XPO1 antibody, Xpo1 antibody, crm1 antibody, dCRM1 antibody, l(2)k16715 antibody, 18.m06600 antibody, DDBDRAFT_0183812 antibody, DDBDRAFT_0234066 antibody, DDB_0183812 antibody, DDB_0234066 antibody, exportin-1 antibody, LOC100220104 antibody, emb antibody, exp1 antibody, Xpo antibody, AA420417 antibody, Exp1 antibody, embargoed antibody, exportin 1 antibody, putative exportin 1 antibody, chromosome region maintenance protein 1 antibody, exportin-1 antibody, exportin 1 (CRM1 homolog, yeast) antibody, exportin 1 S homeolog antibody, emb antibody, cgd3_3060 antibody, Tc00.1047053511725.150 antibody, Tb11.01.5940 antibody, BBOV_II007220 antibody, LMJF_32_1100 antibody, xpo1 antibody, Xpo1 antibody, XPO1 antibody, LOC100165622 antibody, LOC100633509 antibody, xpo1.S antibody

Background: The nuclear pore complex (NPC) is a 125 MDa nuclear envelope complex that mediates nucleocytoplasmic shuttling of RNA and protein. NPC is comprised of more than 30 different proteins, including Ran/TC4, importins, and exportins . Exportin-1(hCRM1/XPO1) is the widely expressed human homologue of the yeast CRM1 protein, which functions in the maintenance of chromatin structure and nuclear export. Exportin-1 has an N-terminal CRIME domain that is homologous to yeast CRM1 and importin-beta. Intracellular localization of Exportin-1 shows association with the NPC and overexpression of Exportin-1 in Xenopus oocytes stimulates Rev and U snRNA export from the nucleus. Exportin-1 also binds other nuclear transport related proteins, such as RanGTP, snurportin-1, and the nucleoporins CAN/Nup214 and Nup50. Overexpression of CAN leads to sequestration of Exportin-1 in the nucleoplasm, which inhibits cell growth and induces apoptosis. In addition, Exportin-1 may export specific proteins, such as Rev and PKI, via binding to a leucine-rich nuclear export signal (NES). Thus, Exportin-1 is an important protein and RNA nuclear export receptor, which may be required for normal cell growth and survival. This antibody is routinely tested by western blot analysis.
Synonyms: CRM1

Molecular Weight: 112 kDa

Pathways: M Phase