PCNT antibody (AA 1692-1814)

Catalog No. ABIN968666

Product Details anti-PCNT Antibody

Target: PCNT (Pericentrin (PCNT))

Binding Specificity: AA 1692-1814

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse pericentrin aa. 1692-1814

Clone: 30-Pericentrin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-PCNT antibody (ABIN968666)

Arellano, Guadiana, Breunig, Rakic, Sarkisian: "Development and distribution of neuronal cilia in mouse neocortex." in: The Journal of comparative neurology, Vol. 520, Issue 4, pp. 848-73, (2012) (PubMed).

Young, Dictenberg, Purohit, Tuft, Doxsey: "Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes." in: Molecular biology of the cell, Vol. 11, Issue 6, pp. 2047-56, (2000) (PubMed).

Doxsey, Stein, Evans, Calarco, Kirschner: "Pericentrin, a highly conserved centrosome protein involved in microtubule organization." in: Cell, Vol. 76, Issue 4, pp. 639-50, (1994) (PubMed).

Target Details for PCNT

Target: PCNT (Pericentrin (PCNT))

Alternative Name: Pericentrin (PCNT Products)

Background: Centrosomes and other microtubule-organizing centers are a diverse group of organelles that nucleate and organize microtubules for many celllular processes, such as mitotic spindle formation, organelle tranpsort, and protein localization. Pericentrin is a protein in the pericentriolar material, a filamentous matrix surrounding centrioles, which can organize microtubule spindle formation. The structure of pericentrin includes multiple alpha-helical domains that form coiled-coil domains separated by non-helical, non-coiled regions. It is found in a 3 MDa-complex that includes gamma-tubulin, a form of tubulin that nucleates microtubule formation at the centrosome. Pericentrin exhibits the highest expression in embryonic mouse kidney, thymus, and liver. Injection of pericentrin antibodies in Xenopus oocytes disrupts mitotic and meiotic divisions and blocks microtubule aster formation. In addition, dynein transports both pericentrin and gamma-tubulin to centrosomes along microtubules in Xenopus oocyte extracts. Thus, the complex of pericentrin and gamma-tubulin may be recruited to the centrosome by dynein, where the complex becomes anchored to the centrosome for microtubule nucleating activity. This antibody is routinely tested by western blot analysis.

Molecular Weight: 220 kDa

Pathways: Sensory Perception of Sound, M Phase, SARS-CoV-2 Protein Interactome