Dynamin 1-Like antibody (AA 19-201)

Catalog No. ABIN968652

This anti-Dynamin 1-Like antibody is a Mouse Monoclonal antibody detecting Dynamin 1-Like in WB and IF. Suitable for Human, Mouse, Rat and Dog. This Primary Antibody has been cited in 1 publication.

Quick Overview for Dynamin 1-Like antibody (AA 19-201) (ABIN968652)

Target: Dynamin 1-Like (DNM1L)

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dynamin 1-Like antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 22-Drp1

Product Details anti-Dynamin 1-Like Antibody

Binding Specificity: AA 19-201

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Drp1 aa. 19-201

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968551, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Dynamin 1-Like antibody (ABIN968652)

Deyo, Chiao, Tainsky: "drp, a novel protein expressed at high cell density but not during growth arrest." in: DNA and cell biology, Vol. 17, Issue 5, pp. 437-47, (1998) (PubMed).

Target Details for Dynamin 1-Like

Target: Dynamin 1-Like (DNM1L)

Alternative Name: Drp1

Background: In culture, cell density can have profound effects on gene expression, enzymatic activity, and cell signaling pathways. Using differential screening of cDNAs from low-passage nontumorigenic teratocarcinoma cells versus high passage tumorigenic cells, a protein was identified that is regulated in cell-density dependent manner. This protein, density-regulated protein 1 (drp1), contains putative sites for N-myristoylation and phosphorylation sites for cAMP and/or cGMP-dependent kinase, casein kinase II, and PKC. The expression of drp1 is enriched in high density cultures of both nontumorigenic and tumorigenic cell lines and is widely detected in adult organs, especially skeletal and cardiac muscle. In addition, increased expression of drp1 is not due to growth arrest as a result of serum starvation or TGF-beta treatment nor is it a result of factors found in the media of high density cultures. Interestingly, drp1 is expressed highest in skeletal and cardiac muscle where unique cell-cell contacts are involved in muscle cell membrane depolarization and contraction. Thus, drp1 expression may be regulated by signaling pathways related to specific types of cell-cell contacts. This antibody is routinely tested by western blot analysis.
Synonyms: Density Regulated Protein-1

Molecular Weight: 27 kDa