Espin antibody (AA 458-580)

Catalog No. ABIN968627

Product Details anti-Espin Antibody

Target: Espin (ESPN)

Binding Specificity: AA 458-580

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Espin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat espin aa. 458-580

Clone: 31-Espin

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Espin antibody (ABIN968627)

Chen, Li, Wang, Wang, Zheng, Bartles: "Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the Sertoli cell-spermatid ectoplasmic specialization junctional plaque." in: Molecular biology of the cell, Vol. 10, Issue 12, pp. 4327-39, (2000) (PubMed).

Bartles, Zheng, Li, Wierda, Chen: "Small espin: a third actin-bundling protein and potential forked protein ortholog in brush border microvilli." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 107-19, (1998) (PubMed).

Bartles, Wierda, Zheng: "Identification and characterization of espin, an actin-binding protein localized to the F-actin-rich junctional plaques of Sertoli cell ectoplasmic specializations." in: Journal of cell science, Vol. 109 ( Pt 6), pp. 1229-39, (1997) (PubMed).

Target Details for Espin

Target: Espin (ESPN)

Alternative Name: Espin (ESPN Products)

Synonyms: DFNB36 antibody, je antibody, Je antibody, espin antibody, ESPN antibody, TVAG_100530 antibody, LOAG_01035 antibody, espn antibody, Espn antibody

Background: Actin-binding proteins regulate the polymerization and depolymerization of actin, connect actin-based structures to membranes and to other cytoskeletal elements, power the movement of actin filaments, and cross-link actin filaments into bundles. Espins are actin binding and bundling proteins. The two isoforms of espin are the 30 kDa small espin found in brush border cells and the 110 kDa espin found in testis. Espin contains eight ankyrin repeats in the N-terminal region, two proline-rich peptides, an ATP/GTP binding P-loop domain, and a C-terminal actin bundling domain. Small espin is composed of the C-terminal actin bundling domain and a unique region at the N-terminus. Espin binds to actin with a higher affinity than small espin and is more efficient at actin bundling. During spermiogenesis, espin accumulates, along with forming parallel actin bundles, at the ectoplasmic specialization. These actin bundles anchor and position the spermatid within the seminiferous epithelium. Other actin binding proteins, such as alpha-actinin, vinculin, and fimbrin, have also been implicated in the formation of ectoplasmic specialization, however espin appears to function specifically in the testes.

Molecular Weight: 110 kDa

Pathways: Sensory Perception of Sound