ALIX antibody (AA 375-580)

Catalog No. ABIN968616

Product Details anti-ALIX Antibody

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Binding Specificity: AA 375-580

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALIX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse AIP1 aa. 375-580

Clone: 49-AIP1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-ALIX antibody (ABIN968616)

Chen, Borinstein, Gillis, Sykes, Bogler: "The glioma-associated protein SETA interacts with AIP1/Alix and ALG-2 and modulates apoptosis in astrocytes." in: The Journal of biological chemistry, Vol. 275, Issue 25, pp. 19275-81, (2000) (PubMed).

Vito, Pellegrini, Guiet, DAdamio: "Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction." in: The Journal of biological chemistry, Vol. 274, Issue 3, pp. 1533-40, (1999) (PubMed).

Target Details for ALIX

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Alternative Name: AIP1 (PDCD6IP Products)

Synonyms: DDBDRAFT_0167145 antibody, DDBDRAFT_0185177 antibody, DDB_0167145 antibody, DDB_0185177 antibody, AIP1 antibody, ALIX antibody, DRIP4 antibody, HP95 antibody, AI480591 antibody, AW544830 antibody, Aip1 antibody, Alix antibody, C76364 antibody, Eig2 antibody, mKIAA1375 antibody, CG12876 antibody, DAIP1 antibody, Dmel\\CG12876 antibody, GB11744 antibody, RGD1561176 antibody, zgc:63638 antibody, wu:fb70d03 antibody, wu:fy65h02 antibody, PDCD6IP antibody, aip1 antibody, alix antibody, hp95 antibody, drip4 antibody, ALG-2 interacting protein X antibody, programmed cell death 6 interacting protein antibody, programmed cell death 6-interacting protein antibody, Programmed cell death 6 interacting protein antibody, programmed cell death 6 interacting protein L homeolog antibody, alxA antibody, PDCD6IP antibody, Pdcd6ip antibody, ALiX antibody, LOC412662 antibody, pdcd6ip antibody, Bm1_48390 antibody, pdcd6ip.L antibody

Background: Apoptosis is a selective process of genetically programmed cell death which occurs during normal cell differentiation and development of multicellular organisms. In vertebrates, T cell and neuronal development are probably the best characterized systems for the study of apoptosis. ALG-2 and ALG-3 (apoptosis-linked genes 2 and 3) were identified as low molecular weight Ca2+-binding proteins essential for apoptosis through the activation of the Fas receptor in T cells. ALG-2 Interacting Protein 1 (AIP1/Alix) is a ubiquitous protein that associates with ALG-2 in the cytosol in a Ca2+ dependent manner. AIP1 is homologous to the yeast protein, BRO1, which has been implicated in Pkc1p- AP kinase signaling. A truncated form of AIP1 protects against serum starvation-, etoposide-, and staurosporine-induced cell death. In addition, the C-terminal proline rich region of AIP1 facilitates interaction with SH3 domain-containing protein expressed in tumorigenic astrocytes (SETA) and this interaction may be important for mediating DNA damage-dependent apoptosis in astrocytes. Thus, AIP1 interacts with ALG-2 or SETA, or both, during activation of cell death pathways in a variety of cell types.
Synonyms: ALG-2 Interacting Protein 1, Alix

Molecular Weight: 105 kDa

Pathways: p53 Signaling, EGFR Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Tube Formation