ARHGAP5 antibody (AA 1102-1214)

Catalog No. ABIN968611

Product Details anti-ARHGAP5 Antibody

Target: ARHGAP5 (rho GTPase Activating Protein 5 (ARHGAP5))

Binding Specificity: AA 1102-1214

Reactivity: Human, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARHGAP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p190-B aa. 1102-1214

Clone: 54-P190

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-ARHGAP5 antibody (ABIN968611)

Burbelo, Finegold, Kozak, Yamada, Takami: "Cloning, genomic organization and chromosomal assignment of the mouse p190-B gene." in: Biochimica et biophysica acta, Vol. 1443, Issue 1-2, pp. 203-10, (1999) (PubMed).

Burbelo, Miyamoto, Utani, Brill, Yamada, Hall, Yamada: "p190-B, a new member of the Rho GAP family, and Rho are induced to cluster after integrin cross-linking." in: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 30919-26, (1996) (PubMed).

Target Details for ARHGAP5

Target: ARHGAP5 (rho GTPase Activating Protein 5 (ARHGAP5))

Alternative Name: p190-B (ARHGAP5 Products)

Synonyms: gfi2 antibody, p190-b antibody, rhogap5 antibody, p190brhogap antibody, GFI2 antibody, RhoGAP5 antibody, p190-B antibody, p190BRhoGAP antibody, p190B antibody, LRRGT00098 antibody, Rho GTPase activating protein 5 antibody, ARHGAP5 antibody, arhgap5 antibody, Arhgap5 antibody

Background: GTPase activating proteins (GAPs) stimulate the GTP-hydrolyzing activity of GTPases, such as p21ras and Rho. p190-A is a Ras-GAP associated protein that is tyrosine phosphorylated in transformed and growth factor-stimulated cells. Ras-GAP and p190-A are targets of oncoproteins and growth factor receptors. p190-B is another Ras-GAP in the p190 family. It has 51% identity with p190-A and contains several GTPase-related domains in the N-terminal region and a Rho-GAP domain in the C-terminal region. p190-B is expressed in kidney, brain, liver, and lung, as well as in human foreskin fibroblasts, RD muscle cells, and HT-1080 cells. In fibroblasts, p190-B is localized diffusely in the cytoplasm and co-localizes with the alpha5beta1 integrin receptor for fibronectin. Adhesion of fibronectin-coated latex beads to cells leads to the recruitment of p190-B and Rho to the plasma membrane at sites of bead contact. In addition, the recombinant Rho-GAP domain of p190-B displays GAP activity for RhoA, Rac1, and G25K/CDC42Hs. Thus, p190-B is thought to act as a transmembrane link between integrins and Rho GTPases during fibronectin-induced changes in cell morphology and motility.

Molecular Weight: 190 kDa

Pathways: Regulation of Cell Size