Integrin beta 4 antibody (AA 1612-1821)

Catalog No. ABIN968433

Product Details anti-Integrin beta 4 Antibody

Target: Integrin beta 4 (ITGB4)

Binding Specificity: AA 1612-1821

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Integrin beta4 aa. 1612-1821

Clone: 7-CD104

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Integrin beta 4 antibody (ABIN968433)

Shaw, Rabinovitz, Wang, Toker, Mercurio: "Activation of phosphoinositide 3-OH kinase by the alpha6beta4 integrin promotes carcinoma invasion." in: Cell, Vol. 91, Issue 7, pp. 949-60, (1998) (PubMed).

Mainiero, Pepe, Wary, Spinardi, Mohammadi, Schlessinger, Giancotti: "Signal transduction by the alpha 6 beta 4 integrin: distinct beta 4 subunit sites mediate recruitment of Shc/Grb2 and association with the cytoskeleton of hemidesmosomes." in: The EMBO journal, Vol. 14, Issue 18, pp. 4470-81, (1995) (PubMed).

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: CD104 (ITGB4 Products)

Synonyms: AA407042 antibody, C230078O20 antibody, CD104 antibody, integrin subunit beta 4 antibody, integrin beta 4 antibody, ITGB4 antibody, Itgb4 antibody

Background: Cell adhesion to extracellular matrix components or to cell surface proteins, especially those expressed by leukocytes and endothelial cells, is mediated by integrins. Integrins contain noncovalently associated alpha and beta subunits. At least 17 alpha and 8 beta subunits have been identified and these proteins can heterodimerize to form 22 different receptors. The alpha6beta4 integrin is a receptor for various laminins and binds with the highest affinity to laminins 4 and 5. It exhibits elevated expression in the basal cell layer of stratified epithelia, in Schwann cells at the onset of myelination, and in CD4-CD8- pre-T lymphocytes entering the thymus. In addition, alpha6beta4 expression is increased in squamous carcinomas where it promotes invasion through a targeting of PI3 kinase activity. The majority of beta4 comprises a cytoplasmic domain with unique signaling properties. The C-terminal portion of this domain contains two pairs of type III fibronectin-like motifs (FNIII) and a tyrosine activation motif (TAM). Additional domains in the cytoplasmic tail bind Shc and activate the MAPK pathway. Thus, integrin beta4 is an integrin subunit that is important for cell survival, growth, and differentiation. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin beta4

Molecular Weight: 200 kDa

Pathways: Integrin Complex