TIMM23 antibody (AA 5-126)

Catalog No. ABIN968428

This Mouse Monoclonal antibody specifically detects TIMM23 in WB and IF. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for TIMM23 antibody (AA 5-126) (ABIN968428)

Target: TIMM23 (Translocase of Inner Mitochondrial Membrane 23 Homolog (Yeast) (TIMM23))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMM23 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 32-Tim23

Product Details anti-TIMM23 Antibody

Binding Specificity: AA 5-126

Cross-Reactivity: Mouse (Murine), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Tim23 aa. 5-126

Isotype: IgG2a

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TIMM23 antibody (ABIN968428)

Moro, Sirrenberg, Schneider, Neupert, Brunner: "The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix." in: The EMBO journal, Vol. 18, Issue 13, pp. 3667-75, (1999) (PubMed).

Rassow, Dekker, van Wilpe, Meijer, Soll: "The preprotein translocase of the mitochondrial inner membrane: function and evolution." in: Journal of molecular biology, Vol. 286, Issue 1, pp. 105-20, (1999) (PubMed).

Ryan, Leung, Jensen: "Characterization of the mitochondrial inner membrane translocase complex: the Tim23p hydrophobic domain interacts with Tim17p but not with other Tim23p molecules." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 178-87, (1998) (PubMed).

Target Details for TIMM23

Target: TIMM23 (Translocase of Inner Mitochondrial Membrane 23 Homolog (Yeast) (TIMM23))

Alternative Name: Tim23

Background: Mitochondria, the site of cellular energy production, must import all proteins necessary for their function. Import is mediated by two mechanisms: the translocase of the outer membrane (Tom) and the translocase of the inner membrane (Tim). Tim23 and Tim17 are integral membrane proteins that associate to form the import channel for mitochondrial preproteins that contain N-terminal hydrophilic sequences. They also associate with Tim44, an adaptor for the membrane binding of mtHsp70, a matrix heat shock protein, which drives the import of the processed preprotein. The N-terminal intermembrane space domain of Tim23 contains a leucine zipper motif and mediates the formation of a Tim23 dimer. As an imported protein passes through the TOM machinery, its N-terminal matrix targeting sequence interacts with the Tim23 dimer. This induces the dissociation of the dimer and initiation of inner membrane translocation of the presequence. In addition to its 9 kDa N-terminal hydrophilic segment, Tim23 contains a 14 kDa hydrophobic domain with four predicted membrane spans. Thus, Tim23 is an important integral membrane component of the mitochondrial protein translocation machinery.

Molecular Weight: 23 kDa