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TIMM23 antibody (AA 5-126)

TIMM23 Reactivity: Human, Mouse, Rat WB, IF Host: Mouse Monoclonal 32-Tim23 unconjugated
Catalog No. ABIN968428
  • Target See all TIMM23 Antibodies
    TIMM23 (Translocase of Inner Mitochondrial Membrane 23 Homolog (Yeast) (TIMM23))
    Binding Specificity
    • 13
    • 7
    • 2
    • 1
    • 1
    AA 5-126
    Reactivity
    Human, Mouse, Rat
    Host
    • 18
    Mouse
    Clonality
    • 18
    Monoclonal
    Conjugate
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    This TIMM23 antibody is un-conjugated
    Application
    • 18
    • 15
    • 4
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Mouse (Murine), Human
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat Tim23 aa. 5-126
    Clone
    32-Tim23
    Isotype
    IgG2a
    Top Product
    Discover our top product TIMM23 Primary Antibody
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Moro, Sirrenberg, Schneider, Neupert, Brunner: "The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix." in: The EMBO journal, Vol. 18, Issue 13, pp. 3667-75, (1999) (PubMed).

    Rassow, Dekker, van Wilpe, Meijer, Soll: "The preprotein translocase of the mitochondrial inner membrane: function and evolution." in: Journal of molecular biology, Vol. 286, Issue 1, pp. 105-20, (1999) (PubMed).

    Ryan, Leung, Jensen: "Characterization of the mitochondrial inner membrane translocase complex: the Tim23p hydrophobic domain interacts with Tim17p but not with other Tim23p molecules." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 178-87, (1998) (PubMed).

  • Target
    TIMM23 (Translocase of Inner Mitochondrial Membrane 23 Homolog (Yeast) (TIMM23))
    Alternative Name
    Tim23 (TIMM23 Products)
    Synonyms
    Tim23 antibody, TIM23 antibody, tim23 antibody, timm23 antibody, timm23b antibody, CHUNP6887 antibody, TIMM23 antibody, translocase of inner mitochondrial membrane 23 antibody, translocase of inner mitochondrial membrane 23 homolog (yeast) antibody, mitochondrial import inner membrane translocase subunit Tim23 antibody, translocase of inner mitochondrial membrane 23 homolog L homeolog antibody, translocase of inner mitochondrial membrane 23 homolog a (yeast) antibody, Timm23 antibody, TIMM23 antibody, LOC101118849 antibody, timm23.L antibody, timm23a antibody, LOC100157391 antibody, LOC486776 antibody
    Background
    Mitochondria, the site of cellular energy production, must import all proteins necessary for their function. Import is mediated by two mechanisms: the translocase of the outer membrane (Tom) and the translocase of the inner membrane (Tim). Tim23 and Tim17 are integral membrane proteins that associate to form the import channel for mitochondrial preproteins that contain N-terminal hydrophilic sequences. They also associate with Tim44, an adaptor for the membrane binding of mtHsp70, a matrix heat shock protein, which drives the import of the processed preprotein. The N-terminal intermembrane space domain of Tim23 contains a leucine zipper motif and mediates the formation of a Tim23 dimer. As an imported protein passes through the TOM machinery, its N-terminal matrix targeting sequence interacts with the Tim23 dimer. This induces the dissociation of the dimer and initiation of inner membrane translocation of the presequence. In addition to its 9 kDa N-terminal hydrophilic segment, Tim23 contains a 14 kDa hydrophobic domain with four predicted membrane spans. Thus, Tim23 is an important integral membrane component of the mitochondrial protein translocation machinery.
    Molecular Weight
    23 kDa
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