VTI1A antibody (AA 114-217)

Catalog No. ABIN968427

Product Details anti-VTI1A Antibody

Target: VTI1A (Vesicle Transport through Interaction with t-SNAREs 1A (VTI1A))

Binding Specificity: AA 114-217

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This VTI1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Human, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Vti1a aa.114-217

Clone: 45-Vti1a

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-VTI1A antibody (ABIN968427)

Chiu, Novikov, Mukherjee, Shields: "A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis." in: The Journal of cell biology, Vol. 159, Issue 4, pp. 637-48, (2002) (PubMed).

Mallard, Tang, Galli, Tenza, Saint-Pol, Yue, Antony, Hong, Goud, Johannes: "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform." in: The Journal of cell biology, Vol. 156, Issue 4, pp. 653-64, (2002) (PubMed).

Shorter, Beard, Seemann, Dirac-Svejstrup, Warren: "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115." in: The Journal of cell biology, Vol. 157, Issue 1, pp. 45-62, (2002) (PubMed).

Advani, Bae, Bock, Chao, Doung, Prekeris, Yoo, Scheller: "Seven novel mammalian SNARE proteins localize to distinct membrane compartments." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10317-24, (1998) (PubMed).

Xu, Wong, Tang, Subramaniam, Zhang, Hong: "A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21783-9, (1998) (PubMed).

Target Details for VTI1A

Target: VTI1A (Vesicle Transport through Interaction with t-SNAREs 1A (VTI1A))

Alternative Name: Vti1a (VTI1A Products)

Synonyms: zgc:109895 antibody, mvti1 antibody, vti1-rp2 antibody, DDBDRAFT_0219803 antibody, DDBDRAFT_0231536 antibody, DDB_0219803 antibody, DDB_0231536 antibody, MMDS3 antibody, MVti1 antibody, VTI1RP2 antibody, Vti1-rp2 antibody, 1110014F16Rik antibody, 1110018K19Rik antibody, 4921537J05Rik antibody, MVti1a antibody, Vti1 antibody, vesicle transport through interaction with t-SNAREs 1A L homeolog antibody, vesicle transport through interaction with t-SNAREs 1A antibody, v-SNARE family protein antibody, vti1a.L antibody, vti1a antibody, VTI1A antibody, vti1A antibody, Vti1a antibody

Background: Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. VTI1a and VTI1b are putative mammalian SNARE proteins identified by sequence comparison with yeast SNAREs. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. Vti1a, a possible t-SNARE, contains a C-terminal hydrophobic domain and several regions that may form coiled-coil structures. It exists in distinct syntaxin 5- and syntaxin 6-containing SNARE complexes within the Golgi apparatus. Inhibition of Vti1a blocks transport of G proteins to the cell surface and results in their accumulation within the Golgi. Thus, Vti1a functions in protein transport within the secretory pathway.

Molecular Weight: 29 kDa