GS28 antibody (AA 3-108)

Catalog No. ABIN968404

Product Details anti-GS28 Antibody

Target: GS28 (GOSR1) (Golgi SNAP Receptor Complex Member 1 (GOSR1))

Binding Specificity: AA 3-108

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GS28 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat GS28 aa. 3-108

Clone: 1-GS28

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-GS28 antibody (ABIN968404)

Loh, Peter, Subramaniam, Hong: "Mammalian Bet3 functions as a cytosolic factor participating in transport from the ER to the Golgi apparatus." in: Journal of cell science, Vol. 118, Issue Pt 6, pp. 1209-22, (2005) (PubMed).

Hamza, Prohaska, Gitlin: "Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 1215-20, (2003) (PubMed).

Muller, Shorter, Newman, Deinhardt, Sagiv, Elazar, Warren, Shima: "Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion." in: The Journal of cell biology, Vol. 157, Issue 7, pp. 1161-73, (2002) (PubMed).

Lanoix, Ouwendijk, Stark, Szafer, Cassel, Dejgaard, Weiss, Nilsson: "Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1." in: The Journal of cell biology, Vol. 155, Issue 7, pp. 1199-212, (2001) (PubMed).

Subramaniam, Loh, Hong: "N-Ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment proteins (SNAP) mediate dissociation of GS28-syntaxin 5 Golgi SNAP receptors (SNARE) complex." in: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25441-4, (1997) (PubMed).

Subramaniam, Peter, Philp, Wong, Hong: "GS28, a 28-kilodalton Golgi SNARE that participates in ER-Golgi transport." in: Science (New York, N.Y.), Vol. 272, Issue 5265, pp. 1161-3, (1996) (PubMed).

Target Details for GS28

Target: GS28 (GOSR1) (Golgi SNAP Receptor Complex Member 1 (GOSR1))

Alternative Name: GS28 (GOSR1 Products)

Synonyms: CG7700 antibody, Dmel\\CG7700 antibody, FSG28 antibody, GOS28 antibody, dgos28 antibody, GOSR1 antibody, gosr1 antibody, zgc:112064 antibody, GOLIM2 antibody, GOS-28 antibody, GOS28/P28 antibody, GS28 antibody, P28 antibody, AI414660 antibody, AI426320 antibody, BB145494 antibody, GOSRI antibody, p28 antibody, Gos28 antibody, v-SNARE antibody, Golgi SNARE, 28 kDa antibody, golgi SNAP receptor complex member 1 antibody, Golgi SNAP receptor complex member 1 antibody, Gos28 antibody, GOSR1 antibody, gosr1 antibody, CpipJ_CPIJ014329 antibody, PAAG_02888 antibody, Tsp_07481 antibody, Gosr1 antibody, gos-28 antibody

Background: Most vesicular fusion events require the function of N-ethylmaleimide-sensitive factor (NSF) and the soluble NSF attachment proteins (SNAPs). Their function is dependent upon the SNAP receptor (SNARE) family of proteins. The specificity of vesicle docking and fusion is mediated by specific interactions between v-SNAREs on vesicles and t-SNAREs on target membranes. GS28 (Golgi SNARE with a size of 28 kDa) is a SNARE that associates with the cis-Golgi and participates in trafficking between the ER and the Golgi and between Golgi compartments. The majority of GS28, the first 230 and 250 aa, is thought to be anchored to the membrane via the C-terminal hydrophobic tail, which is formed by the remaining 20 aa. GS28 and syntaxin 5, another SNARE, exist as a protein complex in the Golgi and this complex is important for the function of both proteins in ER-Golgi transport. The GS28/syntaxin 5 complex can be dissociated by alpha-SNAP and NSF. In addition, GS28 is thought to interact with alpha-SNAP when the GS28/syntaxin 5 complex is dissociated. Thus, GS28 is a SNARE protein that mediates, in complex with syntaxin 5, transport within the Golgi and between Golgi and ER. This antibody is routinely tested by western blot analysis.

Molecular Weight: 28 kDa