SATB1 antibody (AA 550-667)

Catalog No. ABIN968403

Product Details anti-SATB1 Antibody

Target: SATB1 (SATB Homeobox 1 (SATB1))

Binding Specificity: AA 550-667

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SATB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SATB1 aa. 550-667

Clone: 14-SATB1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-SATB1 antibody (ABIN968403)

Skowronska-Krawczyk, Ma, Schwartz, Scully, Li, Liu, Taylor, Tollkuhn, Ohgi, Notani, Kohwi, Kohwi-Shigematsu, Rosenfeld: "Required enhancer-matrin-3 network interactions for a homeodomain transcription program." in: Nature, Vol. 514, Issue 7521, pp. 257-61, (2014) (PubMed).

Beyer, Thabet, Müller, Sadlon, Classen, Lahl, Basu, Zhou, Bailey-Bucktrout, Krebs, Schönfeld, Böttcher, Golovina, Mayer, Hofmann, Sommer, Debey-Pascher, Endl, Limmer, Hippen, Blazar, Balderas, Quast et al.: "Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. ..." in: Nature immunology, Vol. 12, Issue 9, pp. 898-907, (2011) (PubMed).

Alvarez, Yasui, Niida, Joh, Loh, Kohwi-Shigematsu: "The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development." in: Genes & development, Vol. 14, Issue 5, pp. 521-35, (2000) (PubMed).

Cai, Kohwi-Shigematsu: "Intranuclear relocalization of matrix binding sites during T cell activation detected by amplified fluorescence in situ hybridization." in: Methods (San Diego, Calif.), Vol. 19, Issue 3, pp. 394-402, (2000) (PubMed).

de Belle, Cai, Kohwi-Shigematsu: "The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops." in: The Journal of cell biology, Vol. 141, Issue 2, pp. 335-48, (1998) (PubMed).

Liu, Bramblett, Zhu, Lozano, Kobayashi, Ross, Dudley: "The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression." in: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5275-87, (1997) (PubMed).

Dickinson, Joh, Kohwi, Kohwi-Shigematsu: "A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition." in: Cell, Vol. 70, Issue 4, pp. 631-45, (1992) (PubMed).

Target Details for SATB1

Target: SATB1 (SATB Homeobox 1 (SATB1))

Alternative Name: SATB1 (SATB1 Products)

Synonyms: 2610306G12Rik antibody, AW413156 antibody, SATB1 antibody, sb:cb955 antibody, si:ch211-195k18.2 antibody, SATB homeobox 1 antibody, special AT-rich sequence binding protein 1 antibody, SATB homeobox 1a antibody, SATB1 antibody, Satb1 antibody, satb1 antibody, satb1a antibody

Background: Packaging of fully functional eukaryotic chromosomal DNA within the nucleus requires a highly ordered organizational process. Central to this is the formation of nucleosomes, which consist of octameric histone cores with defined segments of chromatin wound around them, and the interaction of nucleosomes with nuclear matrix components. Such specific genomic DNA segments that interact with the nuclear matrix are called scaffold or matrix attachment regions (SARs or MARs). SATB1 (Special AT-rich sequence-Binding protein 1), expressed predominantly in the thymus, is a homeodomain MAR binding protein. It recognizes ATC sequences that consist of stretches of A's, T's, and C's on one DNA strand. These sequences tend to unwind by extensive base-unpairing in response to negative superhelical strain. SATB1 exclusively binds double stranded DNA in the minor groove with minimal base contact and it has a low affinity for supercoiled DNA. In addition, SATB1 is thought to act as a transcriptional repressor. SATB1 is a MAR binding protein that binds to the base of chromatin loops and may be essential to T cell-specific gene regulation. This antibody is routinely tested by western blot analysis.
Synonyms: Special AT-rich sequence-Binding protein-1

Molecular Weight: 106 kDa

Pathways: Caspase Cascade in Apoptosis, Activated T Cell Proliferation