Thrombospondin 2 antibody (AA 173-295)

Catalog No. ABIN968394

Product Details anti-Thrombospondin 2 Antibody

Target: Thrombospondin 2 (THBS2)

Binding Specificity: AA 173-295

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombospondin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Thrombospondin-2 aa. 173-295

Clone: 4-Thrombospondin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Thrombospondin 2 antibody (ABIN968394)

Kyriakides, Zhu, Smith, Bain, Yang, Lin, Danielson, Iozzo, LaMarca, McKinney, Ginns, Bornstein: "Mice that lack thrombospondin 2 display connective tissue abnormalities that are associated with disordered collagen fibrillogenesis, an increased vascular density, and a bleeding diathesis." in: The Journal of cell biology, Vol. 140, Issue 2, pp. 419-30, (1998) (PubMed).

Kyriakides, Zhu, Yang, Bornstein: "The distribution of the matricellular protein thrombospondin 2 in tissues of embryonic and adult mice." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 46, Issue 9, pp. 1007-15, (1998) (PubMed).

LaBell, Milewicz, Disteche, Byers: "Thrombospondin II: partial cDNA sequence, chromosome location, and expression of a second member of the thrombospondin gene family in humans." in: Genomics, Vol. 12, Issue 3, pp. 421-9, (1992) (PubMed).

Target Details for Thrombospondin 2

Target: Thrombospondin 2 (THBS2)

Alternative Name: Thrombospondin-2 (THBS2 Products)

Synonyms: THBS1 antibody, tsp2 antibody, thbs2 antibody, TSP2 antibody, TMEM200A antibody, TSP-2 antibody, Thbs-2 antibody, thrombospondin 2 antibody, thrombospondin 2 L homeolog antibody, thrombospondin 2a antibody, THBS2 antibody, thbs2.L antibody, thbs2a antibody, Thbs2 antibody

Background: Cell adhesion interactions involve large extracellular glycoproteins such as fibronectin, laminins, and thrombospondins. The thrombospondins (TSPs), a small family of secreted glycoproteins, can be separated into two subfamilies based on structural similarity. TSP-1 and TSP-2 are homotrimers of monomeric 145 kDa chains, while TSP-3,4 and 5 are homopentamers of lower MW chains. TSP-1 and TSP-2 interact with several of the same cell surface receptors and have been termed matricellular proteins. However, the functional roles of TSP-1 and TSP-2 are poorly understood. Study of TSP-2 deficient mice has shed some light on the functional role of this protein. Although these mice appear normal, they in fact exhibit a wide variety of irregularities. These include abnormal collagen fiber development which results in fragile skin and highly flexible tendons and ligaments. They also exhibit increased cortical thickness in long bones and abnormal bleeding time. These results, together with the developmental expression pattern of TSP-2, suggest that it functions as a modulator of the cell surface properties of mesenchymal cells which, in turn, affects cell functions such as adhesion and migration. This antibody is routinely tested by western blot analysis.
Synonyms: TSP-2

Molecular Weight: 200 kDa