G3BP1 antibody (AA 210-323)

Catalog No. ABIN968381

Product Details anti-G3BP1 Antibody

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Binding Specificity: AA 210-323

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This G3BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human G3BP aa. 210-323

Clone: 23-G3BP

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968552, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-G3BP1 antibody (ABIN968381)

Gallouzi, Parker, Chebli, Maurier, Labourier, Barlat, Capony, Tocque, Tazi: "A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein: a potential link between signal transduction and RNA stability." in: Molecular and cellular biology, Vol. 18, Issue 7, pp. 3956-65, (1998) (PubMed).

Parker, Maurier, Delumeau, Duchesne, Faucher, Debussche, Dugue, Schweighoffer, Tocque: "A Ras-GTPase-activating protein SH3-domain-binding protein." in: Molecular and cellular biology, Vol. 16, Issue 6, pp. 2561-9, (1996) (PubMed).

Target Details for G3BP1

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Alternative Name: G3BP (G3BP1 Products)

Synonyms: G3BP antibody, HDH-VIII antibody, fj17h05 antibody, zgc:56034 antibody, wu:fj17h05 antibody, g3bp antibody, MGC53271 antibody, G3BP1 antibody, G3bp antibody, RGD1310666 antibody, AI849976 antibody, B430204O07 antibody, C87777 antibody, mKIAA4115 antibody, G3BP stress granule assembly factor 1 antibody, GTPase activating protein (SH3 domain) binding protein 1 antibody, GTPase activating protein (SH3 domain) binding protein 1 L homeolog antibody, Ras-GTPase-activating protein SH3-domain-binding protein antibody, G3BP1 antibody, g3bp1 antibody, g3bp1.L antibody, LOC100304885 antibody, G3bp1 antibody

Background: The Ras proteins are low molecular weight GTPases that play a critical role in the control of proliferation, differentiation, and cytoskeletal organization. RasGAP acts as a negative regulator of Ras, as well as a downstream target of Ras. It participates in the signal transduction cascade through interactions with other signaling proteins. Via its C-terminal domain, RasGAP interacts with Ras, but the N-terminus interacts with other signaling molecules such as p62 and p190. This function of RasGAP is mediated by the presence of SH2, SH3, and PH domains in the N-terminal region. However, some signaling proteins cannot bind to these domains. In an attempt to identify RasGAP-interacting molecules, a GAP SH3-Binding Protein (G3BP) was identified. This protein exhibits sequence homology to the hnRNP superfamily. It contains RNP1 and RNP2 motifs and is found primarily in the cytosol. G3BP interacts with GAP in proliferating cells and is dependent on the activation state of Ras. In addition, G3BP contains an intrinsic endonuclease activity that cleaves 3'-untranslated region (3'-UTR) mRNA. These data indicate that G3BP may link the Ras pathway to mRNA degradation.

Molecular Weight: 55-70 kDa

Pathways: SARS-CoV-2 Protein Interactome