CDH15 antibody (AA 253-366)

Catalog No. ABIN968363

Product Details anti-CDH15 Antibody

Target: CDH15 (M-Cadherin (CDH15))

Binding Specificity: AA 253-366

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDH15 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse M-Cadherin aa. 253-366

Clone: 5-M

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CDH15 antibody (ABIN968363)

Kang, Feinleib, Knox, Ketteringham, Krauss: "Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 7, pp. 3989-94, (2003) (PubMed).

Kaufmann, Kirsch, Irintchev, Wernig, Starzinski-Powitz: "The M-cadherin catenin complex interacts with microtubules in skeletal muscle cells: implications for the fusion of myoblasts." in: Journal of cell science, Vol. 112 ( Pt 1), pp. 55-68, (1999) (PubMed).

Shimoyama, Shibata, Kitajima, Hirohashi: "Molecular cloning and characterization of a novel human classic cadherin homologous with mouse muscle cadherin." in: The Journal of biological chemistry, Vol. 273, Issue 16, pp. 10011-8, (1998) (PubMed).

Kuch, Winnekendonk, Butz, Unvericht, Kemler, Starzinski-Powitz: "M-cadherin-mediated cell adhesion and complex formation with the catenins in myogenic mouse cells." in: Experimental cell research, Vol. 232, Issue 2, pp. 331-8, (1997) (PubMed).

Donalies, Cramer, Ringwald, Starzinski-Powitz: "Expression of M-cadherin, a member of the cadherin multigene family, correlates with differentiation of skeletal muscle cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 18, pp. 8024-8, (1991) (PubMed).

Target Details for CDH15

Target: CDH15 (M-Cadherin (CDH15))

Alternative Name: M-Cadherin (CDH15 Products)

Synonyms: AI323380 antibody, Cdh14 antibody, Mcad antibody, CDH14 antibody, CDH3 antibody, CDHM antibody, MCAD antibody, MRD3 antibody, cadherin 15 antibody, Cdh15 antibody, CDH15 antibody

Background: Cadherins are a family of transmembrane glycoproteins involved in the Ca2+-dependent cell-cell adhesion that occurs in many tissues. Members of this family include P-Cadherin, E-Cadherin (uvomorulin), N-Cadherin, R-Cadherin, Cadherin-5, L-CAM, and EP-Cadherin. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca2+ and protease sensitivity, and molecular weight. However, cadherins have distinct tissue expression patterns and immunological reactivities. M (muscle)-Cadherin, another member of the Cadherin family, was discovered in myogenic mouse cells where it is present at low levels in myoblasts. It is expressed in prenatal and adult skeletal muscle and plays a role in skeletal muscle cell differentiation, particularly the fusion of myoblasts into myotubes. It is upregulated upon induction of myotube formation. M-Cadherin also forms complexes with the catenins in skeletal muscle cells, which then interact with the cytoskeleton. Therefore, it is thought that the M-Cadherin-cytoskeleton interaction may play a role in aligning myoblasts during fusion. This antibody is routinely tested by western blot analysis.

Molecular Weight: 130 kDa