SRPK1 antibody (AA 312-434)

Catalog No. ABIN968353

Product Details anti-SRPK1 Antibody

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Binding Specificity: AA 312-434

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This SRPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SRPK1 aa.312-434

Clone: 12-SRPK1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SRPK1 antibody (ABIN968353)

Wang, Lin, Dyck, Yeakley, Songyang, Cantley, Fu: "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells." in: The Journal of cell biology, Vol. 140, Issue 4, pp. 737-50, (1998) (PubMed).

Colwill, Feng, Yeakley, Gish, Cáceres, Pawson, Fu: "SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24569-75, (1996) (PubMed).

Target Details for SRPK1

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Alternative Name: SRPK1 (SRPK1 Products)

Synonyms: SFRSK1 antibody, AU017960 antibody, BEST:CK01209 antibody, CG11489 antibody, CG9085 antibody, CK01209 antibody, Dmel\\CG11489 antibody, SRPK antibody, SRPK1 antibody, Srpk79D antibody, atc antibody, srpk1 antibody, zgc:63789 antibody, wu:fc06h03 antibody, zgc:158800 antibody, sfrsk1 antibody, SRSF protein kinase 1 antibody, serine/arginine-rich protein specific kinase 1 antibody, Serine-arginine protein kinase at 79D antibody, SRSF protein kinase 1a antibody, SRSF protein kinase 1b antibody, SRSF protein kinase 1 S homeolog antibody, SRPK1 antibody, Srpk1 antibody, Srpk79D antibody, srpk1a antibody, srpk1b antibody, srpk1.S antibody

Background: Mammalian cell pre-mRNA splicing is mediated by the spliceosome, a multi-component complex that contains two types of splicing factors: small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP factors. Interactions between snRNPs and pre-mRNA ensures proper establishment of a catalytic core for the splicing reaction. However, these interactions are mediated by the non-snRNP factors. The super family of Arg/Ser-rich (RS) domain containing splicing factors are well known non-snRNPs. All of these proteins share a similar structure consisting of an N-terminal RNA recognition motif and a C-terminal RS domain. However, different SR factors have distinct specificities and function is regulated by their level of expression and by reversible phosphorylation. Two families of kinases phosphorylate SR domain-containing proteins: SR protein-specific kinases (SRPK1 and 2) and Clk/Sty. SRPL1 is specific for SR domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, whereas SRPK2 is highly expressed in brain. SRPKs affect splice-site selection and are thought to affect alternative splicing.

Molecular Weight: 92 kDa

Pathways: Toll-Like Receptors Cascades