PI3-Kinase p170 antibody

Catalog No. ABIN968340

Product Details

Target: PI3-Kinase p170

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Cross-Reactivity: Rat (Rattus), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse PI3-Kinase p170

Clone: 17-PI3

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Brown, Ho, Weber-Hall, Shipley, Fry: "Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K." in: Biochemical and biophysical research communications, Vol. 233, Issue 2, pp. 537-44, (1997) (PubMed).

Virbasius, Guilherme, Czech: "Mouse p170 is a novel phosphatidylinositol 3-kinase containing a C2 domain." in: The Journal of biological chemistry, Vol. 271, Issue 23, pp. 13304-7, (1996) (PubMed).

Target Details

Target: PI3-Kinase p170

Background: PI3-kinase phosphorylates the D-3 position of the inositol ring of phosphatidylinositol (PtdIns), producing PtdIns(3)P, PtdIns(3,4)P2, and PtdIns(3,4,5)P3. PI3-Kinase is a heterodimer of an 85 kDa regulatory subunit (p85) and a 110 kDa catalytic subunit (p110). However, this heterodimer is only one member of a larger family of proteins with similarity to the p110 subunit. These different PI3-kinase isoforms have been divided into three classes. Class I consists of p110alpha and p110beta which bind the p85 subunit and associate with receptor tyrosine kinases. Class II contains 68D, cpk, and p170. These proteins all contain a C-terminal C2 domain and phosphorylate PtdIns and PtdIns(4)P, but not PtdIns(4,5)P2. Class III members only phosphorylate PtdIns to PtdIns(3)P and include the S. cerevisiae Vps34p and its human homologs. The first human Class II PI3-kinase to be described, p170, contains the characteristic C-terminal phosphoinositide-binding C2 domain also found in PKC isoforms and synaptotagmins. In addition, expression of PI3-kinase p170 is widespread and may indicate that the protein performs a housekeeping function in cell growth and differentiation.

Molecular Weight: 170 kDa