ITGA2 antibody (AA 42-245)

Catalog No. ABIN968317

Product Details anti-ITGA2 Antibody

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Binding Specificity: AA 42-245

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human VLA-2alpha aa.42-245

Clone: 2-CD49b

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-ITGA2 antibody (ABIN968317)

Förster, Mäkela, Wärri, Kietz, Becker, Hultenby, Warner, Gustafsson: "Involvement of estrogen receptor beta in terminal differentiation of mammary gland epithelium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 24, pp. 15578-83, (2002) (PubMed).

Russell, Tofilon: "Radiation-induced activation of nuclear factor-kappaB involves selective degradation of plasma membrane-associated I(kappa)B(alpha)." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3431-40, (2002) (PubMed).

Emsley, King, Bergelson, Liddington: "Crystal structure of the I domain from integrin alpha2beta1." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28512-7, (1997) (PubMed).

Wu, Santoro: "Complex patterns of expression suggest extensive roles for the alpha 2 beta 1 integrin in murine development." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 199, Issue 4, pp. 292-314, (1994) (PubMed).

Target Details for ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Alternative Name: CD49b (ITGA2 Products)

Synonyms: BR antibody, CD49B antibody, GPIa antibody, HPA-5 antibody, VLA-2 antibody, VLAA2 antibody, DX5 antibody, integrin subunit alpha 2 antibody, integrin alpha 2 antibody, ITGA2 antibody, Itga2 antibody

Background: Integrins are a family of dimeric proteins that mediate cell-to-cell and extracellular matrix adhesion. They consist of a large alpha chain that is non-covalently associated with a smaller beta chain which is used to define integrin subfamilies. These molecules exhibit a wide range of expression throughout development and adulthood. VLA-2 (very late antigen), a member of the integrin superfamily, was identified on activated T cells, but has since been reported to be on various cell types. VLA-2 is reported to be a heterodimer of integrin alpha2 (CD49b) and integrin beta1 (CD29) subunits. The alpha2 chain contains a large extracellular domain, a transmembrane domain, and a short cytoplasmic tail. VLA-2 functions as a collagen receptor on platelets and fibroblasts, as well as a collagen and laminin receptor on endothelial and epithelial cells. On activated T cells, VLA-2, like LFA-1, exhibits increased number and affinity of ligand binding. Interactions of these molecules with their extracellular matrix ligands is important for directing effector T cells to their target tissues and to provide co-stimulatory signals. Thus, VLA-2 not only plays an important role in cellular adhesion, but may function in intracellular signal transmission. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin alpha2, VLA-2alpha

Molecular Weight: 150 kDa

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex