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NCF2 antibody (AA 317-469)

NCF2 Reactivity: Human, Mouse WB, IF Host: Mouse Monoclonal 29-p67phox unconjugated
Catalog No. ABIN968245
  • Target See all NCF2 Antibodies
    NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))
    Binding Specificity
    • 15
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 317-469
    Reactivity
    • 48
    • 20
    • 18
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 46
    • 2
    • 1
    Mouse
    Clonality
    • 41
    • 8
    Monoclonal
    Conjugate
    • 17
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NCF2 antibody is un-conjugated
    Application
    • 15
    • 13
    • 13
    • 12
    • 9
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Mouse (Murine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human p67 [phox] aa. 317-469
    Clone
    29-p67phox
    Isotype
    IgG2b
    Top Product
    Discover our top product NCF2 Primary Antibody
  • Comment

    Related Products: ABIN968584, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Ago, Nunoi, Ito, Sumimoto: "Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating" in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33644-53, (1999) (PubMed).

    Benna, Dang, Gaudry, Fay, Morel, Hakim, Gougerot-Pocidalo: "Phosphorylation of the respiratory burst oxidase subunit p67(phox) during human neutrophil activation. Regulation by protein kinase C-dependent and independent pathways." in: The Journal of biological chemistry, Vol. 272, Issue 27, pp. 17204-8, (1997) (PubMed).

    Freeman, Lambeth: "NADPH oxidase activity is independent of p47phox in vitro." in: The Journal of biological chemistry, Vol. 271, Issue 37, pp. 22578-82, (1996) (PubMed).

    Leto, Adams, de Mendez: "Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 22, pp. 10650-4, (1994) (PubMed).

    Leto, Lomax, Volpp, Nunoi, Sechler, Nauseef, Clark, Gallin, Malech: "Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src." in: Science (New York, N.Y.), Vol. 248, Issue 4956, pp. 727-30, (1990) (PubMed).

  • Target
    NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))
    Alternative Name
    p67 phox (NCF2 Products)
    Synonyms
    MGC81877 antibody, zgc:158405 antibody, noxa2 antibody, p67-phox antibody, p67phox antibody, NCF-2 antibody, NOXA2 antibody, P67-PHOX antibody, P67PHOX antibody, Ncf-2 antibody, neutrophil cytosolic factor 2 antibody, neutrophil cytosolic factor 2 L homeolog antibody, neutrophil cytosol factor 2 antibody, hypothetical protein antibody, NCF2 antibody, ncf2.L antibody, ncf2 antibody, p67phox antibody, VDBG_06643 antibody, PGTG_05816 antibody, LOC100136240 antibody, Ncf2 antibody
    Background
    The neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. The active site of this enzyme is located in an integral membrane cytochrome, b558, that consists of the two subunits gp91 [phox] and p21 [phox]. Superoxide production depends on the formation of a complex that includes p67 [phox], p47 [phox], and the GTP-binding protein Rac. Upon activation, these proteins translocate from the cytosol to the membrane where they assemble with b558 and induce oxidase activity. p67 [phox] contains two SH3 domains and binds, via its C-terminal SH3 domain, to the proline rich region of p47 [phox]. This binding allows p67 [phox] to indirectly associate with the oxidase. It is thought that the phosphorylated forms of p67 [phox] and p47 [phox] interact and that the phosphorylation of p67 [phox] is regulated by both PKC-dependent and independent pathways. Although the role of p67 [phox] in electron flow control is poorly understood, it is thought that it regulates the transfer of electrons from NADPH to reduce flavin.
    Molecular Weight
    67 kDa
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