DIAPH1 antibody (AA 41-153)

Catalog No. ABIN968212

Product Details anti-DIAPH1 Antibody

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Binding Specificity: AA 41-153

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This DIAPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Rat (Rattus), Human, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse p140mDia aa. 41-153

Clone: 51-mDia1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-DIAPH1 antibody (ABIN968212)

Li, Higgs: "The mouse Formin mDia1 is a potent actin nucleation factor regulated by autoinhibition." in: Current biology : CB, Vol. 13, Issue 15, pp. 1335-40, (2003) (PubMed).

Pennisi: "The architecture of hearing." in: Science (New York, N.Y.), Vol. 278, Issue 5341, pp. 1223-4, (1997) (PubMed).

Watanabe, Madaule, Reid, Ishizaki, Watanabe, Kakizuka, Saito, Nakao, Jockusch, Narumiya: "p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin." in: The EMBO journal, Vol. 16, Issue 11, pp. 3044-56, (1997) (PubMed).

Target Details for DIAPH1

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Alternative Name: p140mDia (DIAPH1 Products)

Synonyms: DIAPH1 antibody, dia1 antibody, drf1 antibody, dfna1 antibody, diap1 antibody, lfhl1 antibody, DIAPH2 antibody, Diap1 antibody, D18Wsu154e antibody, Dia1 antibody, Diaph1 antibody, Drf1 antibody, mKIAA4062 antibody, p140mDia antibody, DFNA1 antibody, DIA1 antibody, DRF1 antibody, LFHL1 antibody, hDIA1 antibody, diaphanous related formin 1 antibody, diaphanous related formin 2 antibody, diaphanous-related formin 1 antibody, protein diaphanous homolog 1 antibody, DIAPH1 antibody, diaph1 antibody, DIAPH2 antibody, Diaph1 antibody, LOC100457442 antibody

Background: GTPases like Ras, Rho, cdc42Hs, and Rac modulate a multitude of cellular functions like cytoskeletal architecture, growth, motility, and gene expression. The activity of the GTP-binding proteins is regulated by factors that accelerate GTP-binding (GAPs) and proteins that enhance the rate of GTP hydrolysis. mDia1, also known as p140mDia and Drf1, is the mammalian homologue of Drosophila's diaphanous, a protein essential for cytokinesis. The 1255 amino acid mDia1 is widely expressed, with a Rho binding domain at the NH2-terminal region, a central polyproline region, and an FH2 domain. This protein also shares homology with the yeast Bn1p essential for budding and the mouse formin necessary for proper limb development. mDia1 binds to GTP-Rho and to the actin-binding protein profilin-all three proteins are co-localized at the lamellipodia in cultured cells. The overexpression of mDia1 promoted the formation of actin filaments, implicating this protein in cell motility events regulated by Rho. mDia1 was also found consistently mutated in familial deafness.
Synonyms: p140mDia, Drf1

Molecular Weight: 140 kDa

Pathways: Sensory Perception of Sound