Flotillin 1 antibody (AA 312-428)

Catalog No. ABIN968194

Product Details anti-Flotillin 1 Antibody

Target: Flotillin 1 (FLOT1)

Binding Specificity: AA 312-428

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Flotillin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Chicken, Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Flotillin aa. 312-428

Clone: 18-Flotillin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Flotillin 1 antibody (ABIN968194)

Yamamori, Itakura, Sugaya, Katsumata, Sakagami, Takahashi: "Differential expression of SNAP-25 family proteins in the mouse brain." in: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, (2011) (PubMed).

Cabin, Shimazu, Murphy, Cole, Gottschalk, McIlwain, Orrison, Chen, Ellis, Paylor, Lu, Nussbaum: "Synaptic vesicle depletion correlates with attenuated synaptic responses to prolonged repetitive stimulation in mice lacking alpha-synuclein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 20, pp. 8797-807, (2002) (PubMed).

Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Morrow, Rea, Martin, Prior, Prohaska, Hancock, James, Parton: "Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48834-41, (2002) (PubMed).

Rybin, Xu, Lisanti, Steinberg: "Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41447-57, (2001) (PubMed).

Target Details for Flotillin 1

Target: Flotillin 1 (FLOT1)

Alternative Name: Flotillin-1 (FLOT1 Products)

Synonyms: reggie-2 antibody, flot1 antibody, Rareg antibody, flot1c antibody, flotillin-1 antibody, flotillin1 antibody, flot1a antibody, reggie2 antibody, si:dz44o19.1 antibody, wu:fj45g11 antibody, flotillin 1 antibody, flotillin-1 antibody, putative flotillin-1 antibody, flotillin 1 S homeolog antibody, flotillin 1 L homeolog antibody, flotillin 1a antibody, FLOT1 antibody, Flot1 antibody, flot1 antibody, LOC5566722 antibody, CpipJ_CPIJ008623 antibody, Smp_016200.1 antibody, flot1.S antibody, flot1.L antibody, flot1a antibody

Background: Caveolae are specialized membrane invaginations of 50-100 nm present in all cells, but abundant in endothelium, muscle cells, and adipocytes. These plasma membrane microdomains function in transcytosis of macromolecules, and are the sites of potocytosis, where small molecules are concentrated and transferred inside the cells by glycosylphosphatidylinositol (GPI)-linked receptors. Caveolin, a 22kDa protein and a well known marker for these plasma membrane microdomains, plays a structural role in these specializations. Flotillin-1 was isolated from the Triton X-100 insoluble buoyant fraction, characteristic of caveolae. Although the mRNA expression of both Flotillin-1 and Caveolin is very similar, Caveolin is undetectable in brain, while Flotillin-1 is very abundant. Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/Flotillin-2), which also colocalizes in the caveolae. Thus, Flotillin-1 and its relative ESA/Flotillin-2 are now incorporated into the expanding list of proteins co-localized at the caveolae which includes PKCalpha, Ras, Rap Src-like kinases, Galphaßgamma,and GPI-linked receptors. This antibody is routinely tested by western blot analysis.

Molecular Weight: 48 kDa