TFCP2 antibody (AA 205-414)

Catalog No. ABIN968193

Product Details anti-TFCP2 Antibody

Target: TFCP2 (Transcription Factor CP2 (TFCP2))

Binding Specificity: AA 205-414

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFCP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LSF aa. 205-414

Clone: 14-LSF

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968551, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TFCP2 antibody (ABIN968193)

Volker, Rameh, Zhu, DeCaprio, Hansen: "Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity." in: Genes & development, Vol. 11, Issue 11, pp. 1435-46, (1997) (PubMed).

Shirra, Zhu, Huang, Pallas, Hansen: "One exon of the human LSF gene includes conserved regions involved in novel DNA-binding and dimerization motifs." in: Molecular and cellular biology, Vol. 14, Issue 8, pp. 5076-87, (1994) (PubMed).

Target Details for TFCP2

Target: TFCP2 (Transcription Factor CP2 (TFCP2))

Alternative Name: LSF (TFCP2 Products)

Background: LSF (alphaCP2 or LBP-1c) is a transcription factor originally identified by its binding to, and induction of, the SV40 late promoter. LSF displays some similarity to the Elf-1/NTF transcription factors of Drosophila. It is primarily found in its dimeric form in solution, however it is the tetrameric form that recognizes a center-to-center 10 bp repeat. LSF-1D (LBP-1d) is the product of an alternative splicing event, resulting in a deletion of a 51 amino acid segment that corresponds with the DNA-binding region of LSF. LSF-1D is found in the cytoplasm, not in the nucleus, and can interact with LSF to form a heterotetramer that can interfere with LSF DNA binding. In mitogen-stimulated human peripheral T cells, LSF is phosphorylated on amino acid 291, which increases its DNA binding activity. Thus, LSF-mediated gene transcription is regulated by its phosphorylation and/or its interaction with LSF-1D during cell growth. This antibody is routinely tested by western blot analysis.
Synonyms: alphaCP2, LBP-1c

Molecular Weight: 63 kDa