TFCP2 antibody (AA 205-414)
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- Target See all TFCP2 Antibodies
- TFCP2 (Transcription Factor CP2 (TFCP2))
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Binding Specificity
- AA 205-414
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Reactivity
- Human, Mouse, Rat, Dog
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TFCP2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Cross-Reactivity
- Mouse (Murine), Rat (Rattus), Dog (Canine)
- Characteristics
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human LSF aa. 205-414
- Clone
- 14-LSF
- Isotype
- IgG1
- Top Product
- Discover our top product TFCP2 Primary Antibody
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- Comment
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Related Products: ABIN968551, ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store undiluted at -20° C.
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Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity." in: Genes & development, Vol. 11, Issue 11, pp. 1435-46, (1997) (PubMed).
: "One exon of the human LSF gene includes conserved regions involved in novel DNA-binding and dimerization motifs." in: Molecular and cellular biology, Vol. 14, Issue 8, pp. 5076-87, (1994) (PubMed).
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Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity." in: Genes & development, Vol. 11, Issue 11, pp. 1435-46, (1997) (PubMed).
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- Target
- TFCP2 (Transcription Factor CP2 (TFCP2))
- Alternative Name
- LSF (TFCP2 Products)
- Synonyms
- cp2 antibody, lsf antibody, sef antibody, lbp-1c antibody, tfcp2c antibody, MGC75945 antibody, TFCP2 antibody, lbp1c antibody, lsf1d antibody, TCFCP2 antibody, LBP1C antibody, LSF antibody, LSF1D antibody, SEF antibody, TFCP2C antibody, CP-2 antibody, CP2 antibody, D230015P20Rik antibody, LBP-1c antibody, LBP-1d antibody, LBP1 antibody, Tcfcp2 antibody, UBP-1 antibody, transcription factor CP2 antibody, transcription factor CP2 L homeolog antibody, tfcp2 antibody, TFCP2 antibody, tfcp2.L antibody, Tfcp2 antibody
- Background
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LSF (alphaCP2 or LBP-1c) is a transcription factor originally identified by its binding to, and induction of, the SV40 late promoter. LSF displays some similarity to the Elf-1/NTF transcription factors of Drosophila. It is primarily found in its dimeric form in solution, however it is the tetrameric form that recognizes a center-to-center 10 bp repeat. LSF-1D (LBP-1d) is the product of an alternative splicing event, resulting in a deletion of a 51 amino acid segment that corresponds with the DNA-binding region of LSF. LSF-1D is found in the cytoplasm, not in the nucleus, and can interact with LSF to form a heterotetramer that can interfere with LSF DNA binding. In mitogen-stimulated human peripheral T cells, LSF is phosphorylated on amino acid 291, which increases its DNA binding activity. Thus, LSF-mediated gene transcription is regulated by its phosphorylation and/or its interaction with LSF-1D during cell growth. This antibody is routinely tested by western blot analysis.
Synonyms: alphaCP2, LBP-1c - Molecular Weight
- 63 kDa
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