FKBP1A antibody (AA 1-108)

Catalog No. ABIN968189

Product Details anti-FKBP1A Antibody

Target: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Binding Specificity: AA 1-108

Reactivity: Human, Mouse, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This FKBP1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human FKBP12 aa. 1-108

Clone: 8-FKBP12

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968552, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-FKBP1A antibody (ABIN968189)

Chen, Liu, Massague: "Mechanism of TGFbeta receptor inhibition by FKBP12." in: The EMBO journal, Vol. 16, Issue 13, pp. 3866-76, (1997) (PubMed).

Wang, Donahoe, Zervos: "Specific interaction of type I receptors of the TGF-beta family with the immunophilin FKBP-12." in: Science (New York, N.Y.), Vol. 265, Issue 5172, pp. 674-6, (1994) (PubMed).

Target Details for FKBP1A

Target: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Alternative Name: FKBP12 (FKBP1A Products)

Synonyms: 12kDa antibody, FKBP12 antibody, FKBP12-T1 antibody, FKBP12-T2 antibody, Fkbp antibody, Fkbp1 antibody, mFKBP1 antibody, mFKBP12 antibody, FKBP-12 antibody, FKBP-1A antibody, FKBP1 antibody, Rotamase antibody, Fkbp12 antibody, ARABIDOPSIS THALIANA FK506-BINDING PROTEIN 12 antibody, ATFKBP12 antibody, FK506-binding protein 12 antibody, IMMUNOPHILIN antibody, MSJ1.19 antibody, MSJ1_19 antibody, PKC12 antibody, PKCI2 antibody, PPIASE antibody, FKBP1B antibody, Fkbp2 antibody, fj02g10 antibody, zgc:73373 antibody, wu:fj02g10 antibody, FKBP1A antibody, Bmfkbp13 antibody, zgc:103752 antibody, FK506 binding protein 1a antibody, FK506 binding protein 1A antibody, FK506-binding protein antibody, FK506-binding protein 12 antibody, FK506 binding protein 1Aa antibody, FK506 binding protein 1C antibody, FK506-binding protein 59 antibody, FK506 binding protein like antibody, FK506 binding protein 1A L homeolog antibody, FK506 binding protein 1Ab antibody, Fkbp1a antibody, FKBP1A antibody, Fkbp12 antibody, FKBP12 antibody, fkbp1aa antibody, FKBP1C antibody, EHI_180160 antibody, LOC5573443 antibody, Fkbp13 antibody, FKBPL antibody, fkbp1a.L antibody, fkbp1ab antibody

Background: Transforming growth factor-beta binds to the TGFbeta family of heteromeric serine/threonine transmembrane receptors (type I and type II). Following binding of TGFbeta, the type II receptor (TbetaR-II) phosphorylates the type I receptor (TbetaR-I) which, in turn, conveys the signal. Since TbetaR-I and TbetaR-II can interact without the stimulation of TGFbeta, leading to unwanted activation, a regulatory mechanism exists. In a yeast genetic screen, immunophilin FKBP12 was associated with the type I receptor. Studies including co-immunoprecipiation, deletion, and point mutations confirmed this interaction. FKBP12 inhibits TbetaR-II mediated phosphorylation of TbetaR-I, inhibiting activation. FKBP12 binds via its rapamycin/Leu-Pro binding pocket to the Leu-Pro sequence adjacent to the phosphorylation site of TbetaR-I. This interaction is blocked by the addition of macrolides, rapamycin, and FK506. Furthermore, mutations in the binding sites of FKBP12 and TbetaR-I abrogates the binding and results in activation of the receptor without the addition of TGFbeta. Thus, FKBP12 is a regulatory protein for TbetaR-I and TbetaR-II-mediated signaling.
Synonyms: FK506 Binding Protein 1A

Molecular Weight: 14 kDa

Pathways: Negative Regulation of Transporter Activity, Methionine Biosynthetic Process