ARF3 antibody (AA 1-181)

Catalog No. ABIN968175

Product Details anti-ARF3 Antibody

Target: ARF3 (ADP-Ribosylation Factor 3 (ARF3))

Binding Specificity: AA 1-181

Reactivity: Human, Rat, Mouse, Chicken, Dog, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Rat (Rattus), Mouse (Murine), Chicken, Dog (Canine), Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ARF3 aa. 1-181

Clone: 41-ARF3

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-ARF3 antibody (ABIN968175)

Lee, Stevens, Hsu, Tsai, Lee, Moss, Vaughan: "Expression in human endothelial cells of ADP-ribosylation factors, 20-kDa guanine nucleotide-binding proteins involved in the initiation of vesicular transport." in: Journal of molecular and cellular cardiology, Vol. 28, Issue 9, pp. 1911-20, (1997) (PubMed).

Morinaga, Tsai, Moss, Vaughan: "Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 12856-60, (1996) (PubMed).

Cockcroft, Thomas, Fensome, Geny, Cunningham, Gout, Hiles, Totty, Truong, Hsuan: "Phospholipase D: a downstream effector of ARF in granulocytes." in: Science (New York, N.Y.), Vol. 263, Issue 5146, pp. 523-6, (1994) (PubMed).

Moss, Vaughan: "ADP-ribosylation factors, 20,000 M(r) guanine nucleotide-binding protein activators of cholera toxin and components of intracellular vesicular transport systems." in: Cellular signalling, Vol. 5, Issue 4, pp. 367-79, (1993) (PubMed).

Kahn, Radding: "Separation of the presynaptic and synaptic phases of homologous pairing promoted by recA protein." in: The Journal of biological chemistry, Vol. 259, Issue 12, pp. 7495-503, (1984) (PubMed).

Target Details for ARF3

Target: ARF3 (ADP-Ribosylation Factor 3 (ARF3))

Alternative Name: ARF3 (ARF3 Products)

Synonyms: 5430400P17Rik antibody, AI854770 antibody, arf3 antibody, MGC76217 antibody, MGC79549 antibody, ARF3 antibody, ADP-ribosylation factor 3 antibody, ARF-LIKE 1 antibody, ARL1 antibody, ATARL1 antibody, F27A10.8 antibody, ARF1 antibody, si:dz202l16.1 antibody, wu:fc66h11 antibody, zgc:92190 antibody, ADP ribosylation factor 3 antibody, ADP-ribosylation factor 3 antibody, ADP ribosylation factor 1 antibody, ADP ribosylation factor 3 S homeolog antibody, ADP-ribosylation factor 3b antibody, ADP-ribosylation factor 3a antibody, ARF3 antibody, Arf3 antibody, arf1 antibody, arf3.S antibody, arf3b antibody, arf3a antibody

Background: The ADP-ribosylation factors (ARFs) belong to the multigene family of small GTPases capable of activating cholera toxin. ARFs fall into three different classes: Class I is composed of ARF-1, ARF-2, and ARF-3, Class II consists of ARF-4 and ARF-5, and Class III includes ARF6. Unique to ARFs is their lack of intrinsic GTP hydrolysis activity, a high affinity for GDP in a Mg2+-dependent manner, and phospholipid requirement for nucleotide exchange. ARFs are involved in intravesicular acidification of microsomal vesicles, endosome fusion, nuclear membrane assembly, and formation of clathrin-coated vesicles. In addition, GTP and ARF-3 are required for the activation of phospholipase D (PLD), an early cellular response triggered by the binding of receptors on the cell surface in response to numerous extracellular signals. Although predominantly cytosolic, ARF-3 can be translocated to cellular membranes upon cellular stimulation. The transition between the GDP-bound ARF-3 to the GTP-ARF-3 is facilitated by a high molecular weight guanine nucleotide-exchange factor sensitive to brefeldin. ARF-3 has been reported to be the most abundant in brain, kidney, and liver.
Synonyms: ADP Ribosylation Factor-3

Molecular Weight: 20 kDa

Pathways: Inositol Metabolic Process, Cellular Glucan Metabolic Process