CASK antibody (AA 353-486)

Catalog No. ABIN968174

Product Details anti-CASK Antibody

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Binding Specificity: AA 353-486

Reactivity: Human, Mouse, Rat, Dog, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CASK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Human, Dog (Canine), Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat CASK aa. 353-486

Clone: 7-CASK

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CASK antibody (ABIN968174)

Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Biederer, Sudhof: "CASK and protein 4.1 support F-actin nucleation on neurexins." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 47869-76, (2001) (PubMed).

Borg, Lõpez-Figueroa, de Taddèo-Borg, Kroon, Turner, Watson, Margolis: "Molecular analysis of the X11-mLin-2/CASK complex in brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 4, pp. 1307-16, (1999) (PubMed).

Irie, Hata, Takeuchi, Ichtchenko, Toyoda, Hirao, Takai, Rosahl, Südhof: "Binding of neuroligins to PSD-95." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1511-5, (1997) (PubMed).

Hata, Butz, Südhof: "CASK: a novel dlg/PSD95 homolog with an N-terminal calmodulin-dependent protein kinase domain identified by interaction with neurexins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 16, Issue 8, pp. 2488-94, (1996) (PubMed).

Target Details for CASK

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Alternative Name: CASK (CASK Products)

Synonyms: CAKI antibody, CAMGUK antibody, CG13412 antibody, CG13413 antibody, CG6703 antibody, CMG antibody, CMG/CASK antibody, Caki antibody, CamGUK antibody, CamguK antibody, Camguk antibody, Cask antibody, Cmg antibody, DLin-2 antibody, Dmel\\CG6703 antibody, RE09582p antibody, caki antibody, camguk antibody, cmg antibody, dCASK antibody, wu:fb70g06 antibody, CAGH39 antibody, FGS4 antibody, LIN2 antibody, MICPCH antibody, MRXSNA antibody, TNRC8 antibody, DXPri1 antibody, DXRib1 antibody, LIN-2 antibody, Pals3 antibody, mLin-2 antibody, CG6703 gene product from transcript CG6703-RF antibody, Peripheral plasma membrane protein CASK antibody, CAMK/CASK protein kinase antibody, peripheral plasma membrane protein CASK antibody, calcium/calmodulin-dependent serine protein kinase b antibody, calcium/calmodulin-dependent serine protein kinase a antibody, calcium/calmodulin dependent serine protein kinase antibody, calcium/calmodulin-dependent serine protein kinase (MAGUK family) antibody, calcium/calmodulin-dependent serine protein kinase (MAGUK family) S homeolog antibody, CASK antibody, Bm1_33725 antibody, LOAG_17286 antibody, Tsp_03763 antibody, caskb antibody, caska antibody, Cask antibody, cask.S antibody

Background: CASK is a recently identified cytosolic protein kinase with homology to the Ca2+/CaM-dependent kinases and the synaptic associated proteins SAPs/PSDs. Like the SAPs, CASK contains a PDZ domain, an SH3 region, and a guanylate kinase domain. However, unlike the rest of the PDZ protein family, the amino terminus of CASK has significant homology with the Ca2+/Calmodulin-dependent kinases. Although widely expressed, CASK is highly enriched in the synaptic plasma membrane where it associates with neurexins, the neuronal cell surface proteins. Neurexins are a complex family of surface proteins that act as receptors for a number of venoms and toxins and regulate the clustering of several ion channels at the synapse. In addition, neurexins bind heterotypically to neuroligins, therefore adjoining different cell types. Neuroligins bind intracellularly to PSD95 and related proteins, whereas neurexins bind to CASK through their C-terminal region and at CASK's PDZ domain. The interaction of neurexins and CASK at the outside of the cell may modulate CASK's activity and trigger an intracellular signaling cascade.

Molecular Weight: 120 kDa

Pathways: Synaptic Vesicle Exocytosis